a Instituto Nacional de Investigaciones Nucleares (ININ) , Ocoyoacac , México.
b Centro de Protección e Higiene de las Radiaciones (CPHR) , La Habana , Cuba.
Int J Radiat Biol. 2019 May;95(5):607-610. doi: 10.1080/09553002.2019.1566677. Epub 2019 Jan 25.
This paper presents a calibration curve for prematurely condensed chromosome rings (PCC-ring) which are scarce in cytogenetic dosimetry; here we analyzed the behavior of the PCC-ring frequency and PCC index after gamma irradiation in the dose interval from 0 to 20 Gy.
PCC-rings were induced in lymphocytes with Calyculin A. A total of 5788 PCC cells in G1, G2/M, and M/A stages were analyzed.
The best fitting curve between the frequency of PCC ring (Y) and the Dose (D) was obtained with the equation Y = (0.0308 ± 0.0012) D. The distribution of rings by cell followed a Poisson distribution. The PCC index was correlated with radiation dose and decreased in a dose-dependent manner.
This PCC-ring dose-effect curve can be used in case of high dose accidental overexposure to gamma radiation, allowing a dose assessment in a reliable way in most of the simulated irradiation analyzed.
本文提出了一条用于细胞遗传学剂量测定中罕见的过早浓缩染色体环（PCC 环）的校准曲线；在此，我们分析了 PCC 环频率和 PCC 指数在 0 至 20Gy 剂量间隔内伽马辐射后的行为。
用 Calyculin A 诱导淋巴细胞中的 PCC 环。总共分析了 G1、G2/M 和 M/A 期的 5788 个 PCC 细胞。
PCC 环频率（Y）与剂量（D）之间的最佳拟合曲线方程为 Y =（0.0308±0.0012）D。环的分布遵循泊松分布。PCC 指数与辐射剂量相关，并呈剂量依赖性下降。
该 PCC 环剂量效应曲线可用于伽马辐射的高剂量意外超暴露情况，能够在大多数模拟照射分析中可靠地进行剂量评估。
