Translational and Evolutionary Genomics Lab, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India; Department of Biotechnology, Delhi Technological University, Delhi 110042, India.
Translational and Evolutionary Genomics Lab, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
Genomics. 2019 Dec;111(6):1913-1922. doi: 10.1016/j.ygeno.2019.01.002. Epub 2019 Jan 4.
The molecular mechanism of the underlying genes involved in the process of fruit ripening in Capsicum (family Solanaceae) is not clearly known. In the present study, we identified orthologs of 32 fruit development/ripening genes of tomato in Capsicum, and validated their expression in fruit development stages in C. annuum, C. frutescens, and C. chinense. In silico expression analysis using transcriptome data identified a total of 12 out of 32 genes showing differential expression during different stages of fruit development in Capsicum. Real time expression identified gene LOC107847473 (ortholog of MADS-RIN) had substantially higher expression (>500 folds) in breaker and mature fruits, which suggested the non-climacteric ripening behaviour of Capsicum. However, differential expression of Ehtylene receptor 2-like (LOC107873245) gene during fruit maturity supported the climacteric behaviour of only C. frutescens (hot pepper). Furthermore, development of 49 gene based simple sequence repeat (SSR) markers would help in selection of identified genes in Capsicum breeding.
辣椒（茄科）果实成熟过程中涉及的潜在基因的分子机制尚不清楚。本研究在辣椒中鉴定了番茄 32 个果实发育/成熟基因的直系同源物，并验证了它们在 C. annuum、C. frutescens 和 C. chinense 果实发育阶段的表达。使用转录组数据进行的计算机表达分析总共鉴定出 32 个基因中的 12 个在辣椒果实发育的不同阶段表现出差异表达。实时表达鉴定出基因 LOC107847473（MADS-RIN 的直系同源物）在裂果和成熟果实中的表达水平显著较高（>500 倍），这表明辣椒是非跃变型成熟的。然而，乙烯受体 2 样基因（LOC107873245）在果实成熟过程中的差异表达支持了只有 C. frutescens（辣椒）的跃变型行为。此外，基于 49 个基因的简单序列重复（SSR）标记的开发将有助于在辣椒育种中选择鉴定出的基因。
