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Isolation and chemical analysis of 7-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-L-glycero-D-manno-heptose as a constituent of the lipopolysaccharides of the UDP-galactose epimerase-less mutant J-5 of Escherichia coli and Vibrio cholerae.

作者信息

Kaca W, de Jongh-Leuvenink J, Zähringer U, Rietschel E T, Brade H, Verhoef J, Sinnwell V

机构信息

Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, F.R.G.

出版信息

Carbohydr Res. 1988 Aug 15;179:289-99. doi: 10.1016/0008-6215(88)84125-9.

DOI:10.1016/0008-6215(88)84125-9
PMID:3061645
Abstract

Methyl 7-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-L-glycero-D-manno-hepto+ ++ pyranoside (1) was released from the lipopolysaccharide of the UDP-galactose epimerase-less mutant J-5 of Escherichia coli by methanolysis and isolated by high-voltage paper electrophoresis. Its chemical structure was determined by chemical analysis, deamination with nitrous acid, g.1.c.-m.s., and 1H- and 13C-n.m.r. spectroscopy performed on its acetylated derivative. The disaccharide moiety of 1 was also detected in lipopolysaccharides of Vibrio cholerae.

摘要

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