Hisatsune K, Kondo S, Isshiki Y, Iguchi T, Haishima Y
Department of Microbiology, School of Pharmaceutical Sciences, Josai University, Saitama, Japan.
Biochem Biophys Res Commun. 1993 Jan 15;190(1):302-7. doi: 10.1006/bbrc.1993.1046.
A structural study by GC-MS, methylation analysis, and 1H and 13C NMR was carried out on alpha (1-->2)-linked linear N-(3-deoxy-L-glycero-tetronyl)-D-perosamine homopolymer constituting the O-polysaccharide chain of lipopolysaccharide from O1 Vibrio cholerae Ogawa and Inaba O forms. Occurrence of 2-O-methyl-N-(3-deoxy-L-glycero-tetronyl)-D-perosamine was demonstrated at the non-reducing terminus of the perosamine-homopolymer of lipopolysaccharide from the Ogawa O form in contrast to the presence of N-(3-deoxy-L-glycero-tetronyl)-D-perosamine at the nonreducing terminus for the Inaba O form.
采用气相色谱-质谱联用（GC-MS）、甲基化分析以及¹H和¹³C核磁共振（NMR）对构成霍乱弧菌小川型和稻叶型O抗原脂多糖O-多糖链的α(1→2)连接线性N-(3-脱氧-L-甘油-L-四糖基)-D-过氧胺均聚物进行了结构研究。结果表明，小川型O抗原脂多糖过氧胺均聚物的非还原端存在2-O-甲基-N-(3-脱氧-L-甘油-L-四糖基)-D-过氧胺，而稻叶型O抗原脂多糖的非还原端存在N-(3-脱氧-L-甘油-L-四糖基)-D-过氧胺。
