牙周病病原体对 miR-146a 的调控：急性冠脉综合征的风险。
Dysregulation of miR-146a by periodontal pathogens: A risk for acute coronary syndrome.
机构信息
Microbiology Division, Biological Materials Laboratory, CSIR-CLRI, Chennai, India.
Department of Periodontology, Sree Balaji Dental College and Hospital, Chennai, India.
出版信息
J Periodontol. 2019 Jul;90(7):756-765. doi: 10.1002/JPER.18-0466. Epub 2019 Jan 25.
BACKGROUND
Periodontitis is a polymicrobial, chronic inflammatory disease leading to loss of tooth-supporting structures. The bacteremia, endotoxemia, and systemic low-grade inflammation associate periodontitis with systemic illnesses such as diabetes mellitus and coronary artery disease. Periodontal pathogens have been detected from atheromatous plaque by amplification of the genetic material by using specific oligonucleotide primers in polymerase chain reaction. Though the association between periodontitis and cardiovascular diseases has been ascertained by systematic reviews and meta-analyses, its pathophysiology is not lucid. MicroRNAs are currently implicated in the regulation of many cellular processes including inflammation and may play a vital role in our understanding of this disease association. In this case-control study, we explored the role of the inflammatory microRNA, miR-146a, in acute coronary syndrome (ACS) subjects with and without chronic periodontitis (CP) and its regulation of the innate immune host response to periodontal pathogens.
METHODS
Three groups each comprising 66 patients each, namely group 1 (ACS patients without CP), group 2 (ACS patients with CP) and group 3 (CP only) formed the study population. Subgingival plaque samples and serum samples were subjected to quantitative Polymerase Chain Reaction (qPCR) for detection of Porphyromonas gingivalis, a keystone pathogen and to assess the levels of circulating miR-146a and associated proinflammatory cytokines.
RESULTS
miR-146a associated significantly in group 2 subjects with an odds ratio 1.434, 95% confidence interval 1.013-2.030, P < 0.042, and a predictive percentage of 83.3% and group 1 with a predictive percentage of 76.0.% The associated cytokines interleukin-6 (IL-6), tumor necrosis factor-α, and IL-1β also showed an upregulation with statistical significance (P < 0.05).
CONCLUSION
microRNA-146a is a key molecule associating periodontitis with acute coronary syndrome.
背景
牙周炎是一种多微生物、慢性炎症性疾病，导致牙齿支持结构丧失。菌血症、内毒素血症和全身低度炎症使牙周炎与糖尿病和冠状动脉疾病等系统性疾病相关。聚合酶链反应中使用特定的寡核苷酸引物扩增遗传物质，已从动脉粥样斑块中检测到牙周病病原体。尽管系统评价和荟萃分析已经确定了牙周炎与心血管疾病之间的关系，但它的病理生理学并不清楚。MicroRNAs 目前被认为参与包括炎症在内的许多细胞过程的调节，并且可能在我们理解这种疾病关联方面发挥重要作用。在这项病例对照研究中，我们探讨了炎症性 MicroRNA miR-146a 在伴有和不伴有慢性牙周炎（CP）的急性冠状动脉综合征（ACS）患者中的作用及其对牙周病病原体固有免疫宿主反应的调节作用。
方法
本研究纳入了三组各 66 例患者，分别为：第 1 组（无 CP 的 ACS 患者）、第 2 组（有 CP 的 ACS 患者）和第 3 组（仅 CP）。龈下菌斑样本和血清样本经定量聚合酶链反应（qPCR）检测牙龈卟啉单胞菌（一种关键病原体），并评估循环 miR-146a 和相关促炎细胞因子的水平。
结果
miR-146a 在第 2 组患者中与优势比 1.434（95%置信区间 1.013-2.030，P<0.042）显著相关，预测百分比为 83.3%，第 1 组患者的预测百分比为 76.0%。相关细胞因子白细胞介素-6（IL-6）、肿瘤坏死因子-α和白细胞介素-1β也表现出统计学意义的上调（P<0.05）。
结论
miR-146a 是将牙周炎与急性冠状动脉综合征联系起来的关键分子。
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