a Center for Environmental Health Sciences, University of Montana , Missoula , MT , USA.
b Department of Chemistry and Environmental Science , New Jersey Institute of Technology , Newark , NJ , USA.
Inhal Toxicol. 2018 Sep-Oct;30(11-12):429-438. doi: 10.1080/08958378.2018.1547334. Epub 2019 Jan 8.
The toxicity of silver nanomaterials in various forms has been extensively evaluated, but the toxicity of silver nanocarbon composites is less well understood. Therefore, silver-carbon nanotube composites (Ag-MWCNT-COOH) and silver-graphene oxide composites (Ag-GO) were synthesized by microwave irradiation and evaluated in two in vitro cell models.
MATERIALS/METHODS: Toxicity of silver nanosphere (Ag), Ag-MWCNT-COOH and Ag-GO were analyzed by MTS assay and LDH assay in primary C57BL/6 murine alveolar macrophages and human THP-1 cells. Activation of NLRP3 inflammasome by particle variants in these models was done by proxy using LPS co-culture and IL-1β release.
The results depended on the model, as the amount of Ag on the modified carbon resulted in slightly increased toxicity for the murine cells, but did not appear to affect toxicity in the human cell model. IL-1β release from carbon particle-exposures was decreased by the presence of Ag in both cell models. Suspensions of Ag-MWCNT-COOH, Ag-GO and Ag in artificial lysosomal fluid were prepared and ICP-MS was used to detect Ag ions concentration in three silver suspension/solutions. The amount of Ag ions released from Ag-MWCNT-COOH and Ag-GO were similar, which were both lower than that of Ag nanospheres.
The results suggest the bioactivity of silver composites may be related to the amount of Ag ions released, which can be dependent on the cell model under investigation.
各种形式的银纳米材料的毒性已经得到了广泛的评估，但银纳米碳复合材料的毒性却知之甚少。因此，通过微波辐射合成了银-碳纳米管复合材料（Ag-MWCNT-COOH）和银-氧化石墨烯复合材料（Ag-GO），并在两种体外细胞模型中进行了评价。
材料/方法：通过 MTS 分析和 LDH 分析，在原代 C57BL/6 小鼠肺泡巨噬细胞和人 THP-1 细胞中分析了银纳米球（Ag）、Ag-MWCNT-COOH 和 Ag-GO 的毒性。通过 LPS 共培养和 IL-1β释放，以代理方式检测了这些模型中颗粒变体对 NLRP3 炎性体的激活作用。
结果取决于模型，因为修饰碳上的 Ag 量使小鼠细胞的毒性略有增加，但似乎不会影响人细胞模型的毒性。两种细胞模型中，Ag 的存在均降低了碳颗粒暴露引起的 IL-1β释放。在人工溶酶体液中制备了 Ag-MWCNT-COOH、Ag-GO 和 Ag 的悬浮液，并通过 ICP-MS 检测了三种银悬浮液/溶液中 Ag 离子的浓度。Ag-MWCNT-COOH 和 Ag-GO 从 Ag 释放的 Ag 离子的量相似，均低于 Ag 纳米球。
结果表明，银复合材料的生物活性可能与释放的 Ag 离子数量有关，而 Ag 离子的释放量可能取决于所研究的细胞模型。
