Yeh Shin-Joe, Tang Sung-Chun, Tsai Li-Kai, Jeng Jiann-Shing, Chen Chi-Ling, Hsieh Sung-Tsang
Graduate Institute of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei, Taiwan.
Department of Neurology and Stroke Center, National Taiwan University Hospital, Taipei, Taiwan.
Front Behav Neurosci. 2018 Dec 18;12:316. doi: 10.3389/fnbeh.2018.00316. eCollection 2018.
In experimental stroke studies, a neuroanatomy-based functional examination of behaviors is critical to predict the pathological extent of infarcts because brain-imaging studies are not always available. However, there is a lack of systematic studies to examine the efficiency of a behavioral test for this purpose. Our work aimed to design a new score for this goal in stroke rats, by simplifying the Garcia score (with subscore 1-6) and adding circling as subscore 7. MRI and 2,3,5-triphenyltetrazolium chloride staining were used to determine the pathological extent after transient middle cerebral artery occlusion. The modified summations of subscores were designed according to the predictability of each subscore for locations and sizes of infarcts in one group of stroke rats, and were validated in another group. The original Garcia score was able to predict the pathological extent of edema-adjusted infarct size ≥30%, and the summation of subscore 4, 6, and 7 (4: climbing, 6: vibrissae sensation, 7: circling) also could predict it well. The original Garcia score failed to predict infarct at the primary motor cortex, while the summation of subscore 4, 6, and 7 potentially could predict not only the primary motor cortex, but also the forelimb, hindlimb, and barrel field regions of the primary sensory cortex. Accordingly, this neuroanatomy-correlated functional assessment system composed of subscore 4, 6, and 7 was proposed, with less examination time and better inter-rater reliability than the original Garcia score. In summary, this new scoring system, summation (4,6,7) score, examined motor and sensory functions based on neuroanatomical involvement, having the potential to predict the pathological extent and specific relevant brain areas of infarcts, respectively.
在实验性中风研究中，基于神经解剖学的行为功能检查对于预测梗死灶的病理范围至关重要，因为脑成像研究并非总是可行。然而，目前缺乏系统研究来检验用于此目的的行为测试的有效性。我们的工作旨在通过简化加西亚评分（子评分1 - 6）并添加转圈作为子评分7，为中风大鼠设计一个新的评分。使用磁共振成像（MRI）和2,3,5 - 三苯基氯化四氮唑染色来确定短暂性大脑中动脉闭塞后的病理范围。根据一组中风大鼠中每个子评分对梗死灶位置和大小的可预测性设计了子评分的修正总和，并在另一组中进行了验证。原始的加西亚评分能够预测水肿校正后梗死灶大小≥30%的病理范围，子评分4、6和7（4：攀爬，6：触须感觉，7：转圈）的总和也能很好地预测。原始的加西亚评分无法预测初级运动皮层的梗死灶，而子评分4、6和7的总和不仅有可能预测初级运动皮层，还能预测初级感觉皮层的前肢、后肢和桶状区。因此，提出了由子评分4、6和7组成的这种与神经解剖学相关的功能评估系统，与原始的加西亚评分相比，检查时间更短且评分者间信度更高。总之，这个新的评分系统，即总和（4,6,7）评分，基于神经解剖学受累情况检查运动和感觉功能，分别具有预测梗死灶病理范围和特定相关脑区的潜力。
