Schneider Olha, Simic Nebojsa, Aachmann Finn Lillelund, Rückert Christian, Kristiansen Kåre Andre, Kalinowski Jörn, Jiang Yi, Wang Lisong, Jiang Cheng-Lin, Lale Rahmi, Zotchev Sergey B
Department of Biotechnology and Food Science, Norwegian University of Science and Technology, Trondheim, Norway.
Department of Chemistry, Norwegian University of Science and Technology, Trondheim, Norway.
Front Microbiol. 2018 Dec 19;9:3139. doi: 10.3389/fmicb.2018.03139. eCollection 2018.
bacteria are recognized as an important source for antibiotics with broad applications in human medicine and animal health. Here, we report the isolation of a new lichen-associating sp. YIM 130001 from the tropical rainforest in Xishuangbanna (Yunnan, China), which displayed antibacterial activity against . The draft genome sequence of this isolate strain revealed 18 putative biosynthetic gene clusters (BGCs) for secondary metabolites, which is an unusually low number compared to a typical streptomycete. Inactivation of a lantibiotic dehydrogenase-encoding gene from the BGC presumed to govern biosynthesis of a thiopeptide resulted in the loss of bioactivity. Using comparative HPLC analysis, two peaks in the chromatogram were identified in the extract from the wild-type strain, which were missing in the extract from the mutant. The compounds corresponding to the identified peaks were purified, and structure of one compound was elucidated using NMR. The compound, designated geninthiocin B, showed high similarity to several 35-membered macrocyclic thiopeptides geninthiocin, Val-geninthiocin and berninamycin A. Bioinformatics analysis of the geninthiocin B BGC revealed its close homology to that of berninamycins.
细菌被认为是抗生素的重要来源，在人类医学和动物健康领域有广泛应用。在此，我们报告从中国云南西双版纳热带雨林中分离出一种新的地衣共生菌YIM 130001，它对……显示出抗菌活性。该分离菌株的基因组草图序列显示有18个假定的次生代谢物生物合成基因簇（BGC），与典型的链霉菌相比，这个数量异常低。推测负责硫肽生物合成的BGC中一个羊毛硫抗生素脱氢酶编码基因的失活导致了生物活性的丧失。通过比较高效液相色谱分析，在野生型菌株提取物的色谱图中鉴定出两个峰，而突变体提取物中没有这两个峰。对与鉴定出的峰相对应的化合物进行了纯化，并利用核磁共振确定了其中一种化合物的结构。该化合物命名为geninthiocin B，与几种35元大环硫肽geninthiocin、Val-geninthiocin和berninamycin A高度相似。对geninthiocin B BGC的生物信息学分析表明它与berninamycins的BGC密切同源。
