Wachsmuth E D
Research Department, Pharmaceuticals Division, CIBA-GEIGY, Basle, Switzerland.
Histochem J. 1988 Apr;20(4):215-21. doi: 10.1007/BF01747466.
The selective fluorescence staining of two fungi, Candida albicans and Blastomyces dermatitides, with Uvitex 2B and Calcofluor White M2R was studied in deparaffinized and frozen sections of mouse kidney and lung. Both fluorochromes emitted maximally at about 430 nm, independent of the mounting media (Kaiser's gelatin or Entellan). In addition to fungi, both fluorochromes also stained elastic fibres. The fluorescence intensity remained unchanged after storage of sections for more than 6 months in conventional slide boxes. The two fluorochromes showed the following differences: Calcofluor faded 1.25 times faster than Uvitex when illuminated with ultraviolet light. Calcofluor showed a greater affinity for tissues in general, and red cells and renal tubular casts in particular. Counterstaining of deparaffinized sections with Hemalum and Eosin reduced the fungi fluorescence and suppressed the general background fluorescence. However, it led to an intensification of Eosin staining and the fluorescence of red cells in Calcofluor-stained sections but not in Uvitex-stained ones. Similarly, the background fluorescence in frozen sections was reduced by Evans Blue, although elastic fibres still fluoresced after staining with Calcofluor. The degree of staining selectivity, and thus the contrast produced within a histological specimen, was greater with Uvitex 2B than with Calcofluor White M2R.
在小鼠肾脏和肺的石蜡切片和冰冻切片中，研究了用Uvitex 2B和荧光增白剂M2R对白色念珠菌和皮炎芽生菌这两种真菌进行选择性荧光染色的情况。两种荧光染料在约430nm处发射最大荧光，与封片剂（Kaiser明胶或Entellan）无关。除真菌外，两种荧光染料还能对弹性纤维染色。切片在传统载玻片盒中保存6个月以上后，荧光强度保持不变。这两种荧光染料表现出以下差异：在用紫外线照射时，荧光增白剂褪色速度比Uvitex快1.25倍。荧光增白剂一般对组织，尤其是红细胞和肾小管铸型表现出更大的亲和力。用苏木精和伊红对石蜡切片进行复染可降低真菌荧光并抑制总体背景荧光。然而，它导致荧光增白剂染色切片中伊红染色和红细胞荧光增强，但在Uvitex染色切片中则不然。同样，伊文思蓝可降低冰冻切片中的背景荧光，尽管用荧光增白剂染色后弹性纤维仍会发出荧光。Uvitex 2B的染色选择性程度，以及由此在组织学标本中产生的对比度，比荧光增白剂M2R更大。
