Moazeni Maryam, Hedayati Mohammad Taghi, Nabili Mojtaba
Invasive Fungi Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
Department of Medical Mycology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Curr Med Mycol. 2018 Sep;4(3):19-22. doi: 10.18502/cmm.4.3.172.
is a prevalent human fungal pathogen that can cause a wide spectrum of diseases, from superficial mucosal infections to systemic disorders, in patients with impaired immunity. Glabridin is a pyranoisoflavan originally extracted from root extract of . Glabridin can also mediate apoptosis in yeast cells by changing the mitochondrial membrane potential, activation of caspase-like proteases, and DNA cleavage. The aim of this study was to investigate the mechanism of action of glabridin in .
ATCC14053 was applied as the standard strain. Total RNA was extracted from the isolate under glabridin-treated and untreated conditions. To evaluate the alternations in the apoptosis inducing factor () gene expression, real-time polymerase chain reaction (real-time -PCR) was performed, and the obtained data were analyzed using REST software.
Expression of the gene was represented as the ratio of expression relative to the reference gene. According to the REST output, the expression of the gene increased significantly () under the glabridin-treated condition.
Our results suggested that glabridin may induce apoptosis through the caspase-independent route and might be considered as an anti- agent.
是一种常见的人类真菌病原体，可在免疫功能受损的患者中引起从浅表黏膜感染到全身性疾病的广泛疾病。光甘草定是一种最初从的根提取物中提取的吡喃异黄酮。光甘草定还可通过改变线粒体膜电位、激活半胱天冬酶样蛋白酶和DNA裂解来介导酵母细胞凋亡。本研究的目的是探讨光甘草定在中的作用机制。
使用ATCC14053作为标准菌株。在光甘草定处理和未处理条件下从分离株中提取总RNA。为了评估凋亡诱导因子（）基因表达的变化，进行了实时聚合酶链反应（实时-PCR），并使用REST软件分析获得的数据。
基因的表达以相对于参考基因的表达比率表示。根据REST输出，在光甘草定处理条件下，基因的表达显著增加（）。
我们的结果表明，光甘草定可能通过不依赖半胱天冬酶的途径诱导凋亡，并且可能被视为一种抗剂。
