Pekeles Heather, Qureshi Hamid Y, Paudel Hemant K, Schipper Hyman M, Gornistky Mervyn, Chertkow Howard
Departments of Neurology and Neurosurgery and Dentistry, McGill University, Montreal, Québec, Canada.
Lady Davis Institute, Montreal, Québec, Canada.
Alzheimers Dement (Amst). 2018 Apr 21;11:53-60. doi: 10.1016/j.dadm.2018.03.003. eCollection 2019 Dec.
Total tau (t-tau) and phosphorylated tau (p-tau) are abnormally elevated in the brain and cerebrospinal fluid of individuals with Alzheimer's disease (AD). Tau is also present in the salivary gland tissue and saliva, and salivary measures might produce an accurate, accessible, and inexpensive biomarker.
Using unstimulated saliva and Western blot analysis, we quantified the p-tau/t-tau ratio at different phosphorylation sites.
We found that for one phosphorylation site, S396, p-tau/t-tau ratio was significantly elevated in patients with AD compared with normal elderly control subjects. The elevation in saliva, however, did not correlate with cerebrospinal fluid tau or with brain measures such as hippocampal volume.
There is significant elevation of p-tau/t-tau ratio for the S396 phosphorylation site. Large variation in the AD salivary tau levels, however, limits the utility of this test as a clinical biomarker.
在阿尔茨海默病（AD）患者的大脑和脑脊液中，总tau蛋白（t-tau）和磷酸化tau蛋白（p-tau）异常升高。Tau蛋白也存在于唾液腺组织和唾液中，唾液检测可能会产生一种准确、便捷且廉价的生物标志物。
我们使用非刺激性唾液和蛋白质印迹分析，对不同磷酸化位点的p-tau/t-tau比值进行了定量。
我们发现，对于一个磷酸化位点S396，与正常老年对照受试者相比，AD患者的p-tau/t-tau比值显著升高。然而，唾液中的升高与脑脊液tau蛋白或海马体积等脑部测量指标无关。
S396磷酸化位点的p-tau/t-tau比值有显著升高。然而，AD患者唾液tau蛋白水平的巨大差异限制了该检测作为临床生物标志物的实用性。
