乳剂凝聚检测法用于蛋白质-蛋白质相互作用的检测：寨卡病毒光学传感器。
Emulsion Agglutination Assay for the Detection of Protein-Protein Interactions: An Optical Sensor for Zika Virus.
机构信息
Department of Chemistry and Institute for Soldier Nanotechnologies , Massachusetts Institute of Technology , 77 Massachusetts Avenue , Cambridge , Massachusetts 02139 , United States.
Department of Chemical Engineering , Massachusetts Institute of Technology , 77 Massachusetts Avenue , Cambridge , Massachusetts 02139 , United States.
出版信息
ACS Sens. 2019 Jan 25;4(1):180-184. doi: 10.1021/acssensors.8b01202. Epub 2019 Jan 9.
Abstract
A new class of Janus emulsion agglutination assay is reported for the detection of interfacial protein-protein interactions. Janus emulsion droplets are functionalized with a thermally stable, antigen binding protein rcSso7d variant (rcSso7d-ZNS1) for the detection of Zika NS1 protein. The emulsion droplets containing fluorescent dyes in their hydrocarbon and fluorocarbon phases intensify the intrinsic optical signal with the emission intensity ratio, which can be detected by a simple optical fiber. This assay provides an opportunity for the in-field detection of Zika virus and other pathogens with a stable, inexpensive, and convenient device.
摘要
本文报道了一种新型的 Janus 乳状液凝集检测法，用于检测界面蛋白-蛋白相互作用。Janus 乳状液滴用一种热稳定的抗原结合蛋白 rcSso7d 变体（rcSso7d-ZNS1）进行功能化，用于检测寨卡 NS1 蛋白。含有荧光染料的乳状液滴在其碳氢相和氟碳相中的发射强度比增强了固有光学信号，可通过简单的光纤进行检测。该检测法为现场检测寨卡病毒和其他病原体提供了机会，具有稳定、廉价和方便的设备。
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