基于活性的工程化嗜热蛋白作为纸质诊断测试中捕获剂的评估。
Activity-based assessment of an engineered hyperthermophilic protein as a capture agent in paper-based diagnostic tests.
作者信息
Miller E A, Traxlmayr M W, Shen J, Sikes H D
机构信息
Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA, 02139, USA.
出版信息
Mol Syst Des Eng. 2016 Dec 1;1(4):377-381. doi: 10.1039/C6ME00032K. Epub 2016 Jun 29.
Abstract
Antibodies have traditionally served as the affinity reagents of choice in point-of-care diagnostic biosensors. However, this class of proteins is not ideally suited for this use, being poorly characterized and prone to thermal denaturation. Here, we present an activity-based assessment of an alternative engineered binding protein in a cellulose-based assay.
摘要
传统上，抗体一直是即时诊断生物传感器中首选的亲和试剂。然而，这类蛋白质并不完全适合这种用途，其特性不佳且容易发生热变性。在此，我们展示了在基于纤维素的检测中对一种替代工程化结合蛋白的基于活性的评估。
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