利用动态液滴透镜对酶活性进行光学可视化和定量分析。
Optical visualization and quantification of enzyme activity using dynamic droplet lenses.
机构信息
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139.
Institute for Soldier Nanotechnologies, Massachusetts Institute of Technology, Cambridge, MA 02139.
出版信息
Proc Natl Acad Sci U S A. 2017 Apr 11;114(15):3821-3825. doi: 10.1073/pnas.1618807114. Epub 2017 Mar 27.
Abstract
In this paper, we describe an approach to measuring enzyme activity based on the reconfiguration of complex emulsions. Changes in the morphology of these complex emulsions, driven by enzyme-responsive surfactants, modulate the transmission of light through a sample. Through this method we demonstrate how simple photodetector measurements may be used to monitor enzyme kinetics. This approach is validated by quantitative measurements of enzyme activity for three different classes of enzymes (amylase, lipase, and sulfatase), relying on two distinct mechanisms for coupling droplet morphology to enzyme activity (host-guest interactions with uncaging and molecular cleavage).
摘要
本文提出了一种基于复杂乳状液重构的酶活性测量方法。受酶响应性表面活性剂驱动，这些复杂乳状液形态的变化会调节光在样品中的传输。通过这种方法，我们展示了如何使用简单的光电探测器测量来监测酶动力学。该方法通过对三种不同类别的酶（淀粉酶、脂肪酶和磺酸盐酶）的活性进行定量测量得到验证，该方法依赖于两种将液滴形态与酶活性偶联的不同机制（主体-客体相互作用的解笼和分子裂解）。
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