比较牛牙体外侵蚀方案以模拟自然侵蚀病变：机械性能和表面光泽度分析。
Comparison of in vitro erosion protocols in bovine teeth to simulate natural erosion lesion: analysis of mechanical properties and surface gloss.
机构信息
Univ. Estadual Paulista, Faculdade de Odontologia de Araçatuba, Departamento de Odontologia Restauradora, Araçatuba, São Paulo, Brasil.
Univ. Estadual Paulista, Instituto de Ciência e Tecnologia, Departamento de Odontologia Restauradora, São José dos Campos, São Paulo, Brasil.
出版信息
J Appl Oral Sci. 2019 Jan 7;27:e20180107. doi: 10.1590/1678-7757-2018-0107.
OBJECTIVE
The aim of this study was to compare two in vitro erosion protocols, in which one simulates in vivo conditions experienced by patients with gastroesophageal disorders or bulimia (HCl-pepsin protocol), and the other simulates the diet of an individual who consumes a high volume of erosive beverages (citric acid protocol). In addition, the mechanical properties and surface gloss of eroded human dentin were compared with those of sound human dentin.
MATERIALS AND METHODS
Blocks of cervical dentin were used: sound human dentin (n=10), human dentin with erosive lesions (n=10), and bovine dentin (n=30). Twenty bovine blocks were subjected to either of two erosion protocols (n=10/protocol). In the first protocol, samples were demineralized using HCl-pepsin solution, then treated with trypsin solution. In the second protocol, samples were demineralized with 2% citric acid. Toothbrushing was performed in both protocols using a toothbrushing machine (15 s with a 150 g load). Ten bovine dentin blocks were not subjected to any erosive treatment. All samples of bovine and human dentin were analyzed to obtain Martens hardness values (MH), elastic modulus (Eit*) and surface gloss. One-way ANOVA and Tukey's test were performed to analyze the data (α=0.05).
RESULTS
Sound human and eroded human dentin groups showed similar MH and Eit* values (p>0.05); however, sound human dentin showed a higher surface gloss value when compared to eroded human dentin (p<0.05). Sound bovine dentin and HCl-pepsin-treated bovine dentin treatments resulted in similar values for both MH and Eit* (p>0.05), but HCl-pepsin-treated bovine dentin and citric acid-treated bovine dentin resulted in lower surface gloss than sound bovine dentin (p<0.05).
CONCLUSIONS
The HCl-pepsin protocol modified bovine dentin properties that could be similar to those that occur on human dentin surfaces with erosive lesions.
目的
本研究旨在比较两种体外侵蚀方案，一种模拟胃食管疾病或暴食症患者体内经历的条件（HCl-胃蛋白酶方案），另一种模拟大量饮用侵蚀性饮料个体的饮食（柠檬酸方案）。此外，还比较了侵蚀性人牙本质的机械性能和表面光泽度与正常牙本质的差异。
材料和方法
使用颈牙本质块：正常牙本质（n=10）、有侵蚀性病变的牙本质（n=10）和牛牙本质（n=30）。将 20 个牛牙本质块置于两种侵蚀方案中的一种（n=10/方案）。在第一个方案中，使用 HCl-胃蛋白酶溶液对样本进行脱矿处理，然后用胰蛋白酶溶液处理。在第二个方案中，使用 2%柠檬酸对样本进行脱矿处理。两种方案都使用牙刷牙机进行刷牙（15 s，负载 150 g）。十个牛牙本质块未进行任何侵蚀处理。分析所有牛牙本质和人牙本质样本以获得马氏体硬度值（MH）、弹性模量（Eit*）和表面光泽度。采用单因素方差分析和 Tukey 检验进行数据分析（α=0.05）。
结果
正常牙本质和侵蚀牙本质组的 MH 和 Eit*值相似（p>0.05）；然而，正常牙本质的表面光泽度值高于侵蚀牙本质（p<0.05）。正常牛牙本质和 HCl-胃蛋白酶处理的牛牙本质在 MH 和 Eit*方面产生相似的值（p>0.05），但 HCl-胃蛋白酶处理的牛牙本质和柠檬酸处理的牛牙本质的表面光泽度低于正常牛牙本质（p<0.05）。
结论
HCl-胃蛋白酶方案改变了牛牙本质的性质，使其类似于人牙本质表面发生侵蚀性病变时的性质。
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