Department of Maxillofacial Surgery Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo, Tokyo, 113-8549, Japan.
Department of Organic Biomaterials, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo, 101-0062, Japan.
Macromol Biosci. 2019 Apr;19(4):e1800346. doi: 10.1002/mabi.201800346. Epub 2019 Jan 9.
Modulation of material properties and growth factor application are critical in constructing suitable cell culture environments to induce desired cellular functions. Sulfonated polyrotaxane (PRX) surfaces with immobilized vascular endothelial growth factors (VEGFs) are prepared to improve network formation in vascular endothelial cells. Sulfonated PRXs, whereby sulfonated α-cyclodextrins (α-CDs) are threaded onto a linear poly(ethylene glycol) chain capped with bulky groups at both terminals, are coated onto surfaces. The molecular mobility of sulfonated PRX surfaces is modulated by tuning the number of threading α-CDs. VEGF is immobilized onto surfaces with varying mobility. Low mobility and VEGF-immobilization reinforce cell proliferation, yes-associated protein activity, and rhoA, pdgf, ang-1, and pecam-1 gene expression. Highly mobile surfaces and soluble VEGF weakly affect these cell responses. Network formation is strongly stimulated in vascular endothelial cells only on low-mobility VEGF-immobilized surfaces, suggesting that molecular mobility and VEGF immobilization synergistically control cell function.
调控材料性能和生长因子的应用对于构建合适的细胞培养环境以诱导所需的细胞功能至关重要。通过固定血管内皮生长因子（VEGFs）来制备磺化聚轮烷（PRX）表面，以改善血管内皮细胞的网络形成。磺化 PRX 是指磺化的 α-环糊精（α-CDs）被穿在线性聚（乙二醇）链上，两端用大体积基团封端，然后涂覆在表面上。通过调节穿入的 α-CDs 的数量来调节磺化 PRX 表面的分子迁移率。具有不同迁移率的表面固定 VEGF。低迁移率和 VEGF 固定增强细胞增殖、yes 相关蛋白活性以及 rhoA、pdgf、ang-1 和 pecam-1 基因表达。高迁移率的表面和可溶性 VEGF 则对这些细胞反应的影响较弱。只有在低迁移率的 VEGF 固定表面上，才会强烈刺激血管内皮细胞的网络形成，这表明分子迁移率和 VEGF 固定协同控制细胞功能。
