Laboratório de Eletroquímica e Nanotecnologia Aplicada - Instituto de Ciência e Tecnologia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Rodovia MGT 367, Km 583, 5000, Alto da Jacuba, Diamantina, MG 39.100-000, Brazil.
Departamento de Farmácia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Rodovia MGT 367, Km 583, 5000, Alto da Jacuba, Diamantina, MG 39.100-000, Brazil.
Talanta. 2019 Apr 1;195:327-332. doi: 10.1016/j.talanta.2018.11.087. Epub 2018 Nov 23.
Leishmaniasis is a disease with high impact on public health in many countries. Visceral leishmaniasis (VL) is a vectorial zoonosis, with dogs as primary reservoirs in the domestic environment. VL presents high morbidity, mortality and importance in epidemiology in the American continent. In the present study, the first label-free electrochemical impedance immunosensor using screen-printed electrodes (SPEs) for the detection of anti-Leishmania infantum antibodies was developed. The soluble antigens of L. infantum were immobilized on an SPE by a 3-mercaptopropionic acid monolayer. Electrochemical impedance spectroscopy (EIS) was used for detecting bimolecular interactions occurring at the electrode surface. The addition of real samples consisting of canine and human sera positive and negative for VL presented high sensitivity and selectivity through EIS. Based on the results, a sensitive, specific, rapid and simple immunosensor was developed successfully with potential application for the serological diagnosis of leishmaniasis disease.
利什曼病是许多国家公共卫生的一个重要问题。内脏利什曼病（VL）是一种媒介传播的动物传染病，狗是家庭环境中的主要储存宿主。VL 在美洲大陆的发病率、死亡率和流行病学方面具有重要意义。在本研究中，首次开发了基于丝网印刷电极（SPE）的无标记电化学阻抗免疫传感器，用于检测抗利什曼原虫抗体。通过巯基丙酸单层将利什曼原虫的可溶性抗原固定在 SPE 上。电化学阻抗谱（EIS）用于检测发生在电极表面的双分子相互作用。通过 EIS 对由犬和人血清组成的真实样本进行检测，结果显示该方法具有较高的灵敏度和选择性。基于这些结果，成功开发了一种灵敏、特异、快速和简单的免疫传感器，具有用于利什曼病血清学诊断的潜在应用。
