Department of Oriental Medicine Biotechnology, College of Life Sciences and Graduate School of Biotechnology, Kyung Hee University, Global Campus, Gyeonggi 17104, Korea.
Gachon Institute of Pharmaceutical Sciences, College of Pharmacy, Gachon University, Incheon 21936, Korea.
Nutrients. 2019 Jan 8;11(1):113. doi: 10.3390/nu11010113.
Sensorineural hearing loss (SNHL) is one of the most common causes of disability, affecting over 466 million people worldwide. However, prevention or therapy of SNHL has not been widely studied. Avocado oil has shown many health benefits but it has not yet been studied in regards to SNHL. Therefore, we aimed to investigate the efficacy of avocado oil on SNHL in vitro and in vivo and elucidate its mode of action. For the present study, we used enhanced functional avocado oil extract (DKB122). DKB122 led to recovery of otic hair cells in zebrafish after neomycin-induced otic cell damage. Also, DKB122 improved auditory sensory transmission function in a mouse model of noise induced-hearing loss and protected sensory hair cells in the cochlea. In addition, RNA sequencing was performed to elucidate the mechanism involved. KEGG pathway enrichment analysis of differentially expressed genes showed that DKB122 protected House Ear Institute-Organ of Corti 1 (HEI-OC1) cells against neomycin-related alterations in gene expression due to oxidative stress, cytokine production and protein synthesis.
感音神经性听力损失（SNHL）是最常见的残疾原因之一，影响全球超过 4.66 亿人。然而，SNHL 的预防或治疗尚未得到广泛研究。鳄梨油具有许多健康益处，但尚未研究其对 SNHL 的作用。因此，我们旨在研究鳄梨油对体外和体内 SNHL 的疗效，并阐明其作用机制。在本研究中，我们使用了增强型功能性鳄梨油提取物（DKB122）。DKB122 导致新霉素诱导的耳细胞损伤后斑马鱼耳毛细胞的恢复。此外，DKB122 改善了噪声诱导听力损失小鼠模型中的听觉感觉传递功能，并保护了耳蜗中的感觉毛细胞。此外，进行了 RNA 测序以阐明所涉及的机制。差异表达基因的 KEGG 途径富集分析表明，DKB122 可防止 House Ear Institute-Organ of Corti 1（HEI-OC1）细胞因氧化应激、细胞因子产生和蛋白质合成而发生与新霉素相关的基因表达改变。
