Qiu Yan, Huang Jianlin, Jia Li
Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China.
Yunnan Branch of China National Geological Center of Building Materials Industry, Kunming 650118, China.
Int J Anal Chem. 2018 Dec 2;2018:1979684. doi: 10.1155/2018/1979684. eCollection 2018.
A fluorescence sensor for the detection of glutathione based on the fluorescence recovering of the bovine serum albumin-stabilized gold nanoclusters is reported. This study indicates that glutathione restores the copper-ion-quenched fluorescence by coordinating the bound copper ion in the bovine serum albumin molecule used for stabilizing the gold nanocluster through its sulfydryl. Under the experimental conditions, the fluorescence response showed a linear relationship with the concentration of glutathione over the range from 10 M to 400 M. The fluorescence sensor successfully detected glutathione in commercial drug products.
报道了一种基于牛血清白蛋白稳定的金纳米簇荧光恢复来检测谷胱甘肽的荧光传感器。该研究表明，谷胱甘肽通过其巯基与用于稳定金纳米簇的牛血清白蛋白分子中结合的铜离子配位，从而恢复被铜离子淬灭的荧光。在实验条件下，荧光响应在10 M至400 M的谷胱甘肽浓度范围内与谷胱甘肽浓度呈线性关系。该荧光传感器成功检测了市售药品中的谷胱甘肽。
