• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

XPD通过miR-29a-3p-Mdm2/PDGF-B轴抑制肝癌细胞的增殖和迁移。

XPD suppresses cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC.

作者信息

Xiao Zhihua, Wang Yijun, Ding Hao

机构信息

1Department of Gastroenterology, The Second Affiliated Hospital of Nanchang University, 1 Minde Road, Nanchang, 330006 Jiangxi People's Republic of China.

2The Second Clinical Medical College of Nanchang University, Nanchang, 330006 Jiangxi People's Republic of China.

出版信息

Cell Biosci. 2019 Jan 5;9:6. doi: 10.1186/s13578-018-0269-4. eCollection 2019.

DOI:10.1186/s13578-018-0269-4
PMID:30627419
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6321695/
Abstract

OBJECTIVE

The aim of this study was to investigate the role of XPD in migration and invasion of hepatocellular carcinoma (HCC) cells.

METHODS

The expression of XPD and miR-29a-3p was examined by western blot and qRT-PCR, cell proliferation was detected by MTT assay, cell migration was detected by transwell assay. TargetScan was used to predict potential targets of miR-29a-3p.

RESULTS

In this study, we found that the expression of XPD and miR-29a-3p was downregulated in HCC samples and HCC cell lines. XPD suppressed proliferation and migration of HCC cell via regulating miR-29a-3p expression. Target prediction analysis and dual-luciferase reporter assay confirmed Mdm2 and PDGF-B were direct targets of miR-29a-3p, and miR-29a-3p suppressed proliferation and migration of HCC cells via regulating the expression of Mdm2 or PDGF-B.

CONCLUSIONS

Our data indicated that XPD suppressed cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC.

摘要

目的

本研究旨在探讨XPD在肝细胞癌（HCC）细胞迁移和侵袭中的作用。

方法

采用蛋白质免疫印迹法和实时定量聚合酶链反应检测XPD和miR-29a-3p的表达，采用MTT法检测细胞增殖，采用Transwell法检测细胞迁移。利用TargetScan预测miR-29a-3p的潜在靶标。

结果

在本研究中，我们发现HCC样本和HCC细胞系中XPD和miR-29a-3p的表达下调。XPD通过调节miR-29a-3p的表达抑制HCC细胞的增殖和迁移。靶标预测分析和双荧光素酶报告基因检测证实Mdm2和PDGF-B是miR-29a-3p的直接靶标，且miR-29a-3p通过调节Mdm2或PDGF-B的表达抑制HCC细胞的增殖和迁移。

结论

我们的数据表明，XPD在HCC中通过miR-29a-3p-Mdm2/PDGF-B轴抑制细胞增殖和迁移。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ab4/6321695/15ac78e2eda8/13578_2018_269_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ab4/6321695/fa2467b03d11/13578_2018_269_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ab4/6321695/ceec470a7747/13578_2018_269_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ab4/6321695/2888538c3422/13578_2018_269_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ab4/6321695/81f7bb499185/13578_2018_269_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ab4/6321695/f9e7f6c37c74/13578_2018_269_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ab4/6321695/c4e5f3a8d934/13578_2018_269_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ab4/6321695/ec7ad16de263/13578_2018_269_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ab4/6321695/15ac78e2eda8/13578_2018_269_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ab4/6321695/fa2467b03d11/13578_2018_269_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ab4/6321695/ceec470a7747/13578_2018_269_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ab4/6321695/2888538c3422/13578_2018_269_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ab4/6321695/81f7bb499185/13578_2018_269_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ab4/6321695/f9e7f6c37c74/13578_2018_269_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ab4/6321695/c4e5f3a8d934/13578_2018_269_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ab4/6321695/ec7ad16de263/13578_2018_269_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ab4/6321695/15ac78e2eda8/13578_2018_269_Fig8_HTML.jpg

相似文献

1
XPD suppresses cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC.XPD通过miR-29a-3p-Mdm2/PDGF-B轴抑制肝癌细胞的增殖和迁移。
Cell Biosci. 2019 Jan 5;9:6. doi: 10.1186/s13578-018-0269-4. eCollection 2019.
2
COL4A1, negatively regulated by XPD and miR-29a-3p, promotes cell proliferation, migration, invasion and epithelial-mesenchymal transition in liver cancer cells.COL4A1受XPD和miR-29a-3p负调控，促进肝癌细胞的增殖、迁移、侵袭及上皮-间质转化。
Clin Transl Oncol. 2021 Oct;23(10):2078-2089. doi: 10.1007/s12094-021-02611-y. Epub 2021 Apr 23.
3
Exosomal Circ-ZNF652 Promotes Cell Proliferation, Migration, Invasion and Glycolysis in Hepatocellular Carcinoma via miR-29a-3p/GUCD1 Axis.外泌体环状锌指蛋白652通过miR-29a-3p/葡糖糖代谢蛋白1轴促进肝细胞癌的细胞增殖、迁移、侵袭和糖酵解
Cancer Manag Res. 2020 Aug 24;12:7739-7751. doi: 10.2147/CMAR.S259424. eCollection 2020.
4
lncRNA KCNQ1OT1 reverses the effect of sevoflurane on hepatocellular carcinoma progression via regulating the miR-29a-3p/CBX3 axis.长链非编码RNA KCNQ1OT1通过调控miR-29a-3p/CBX3轴逆转七氟醚对肝癌进展的影响。
Braz J Med Biol Res. 2021 May 17;54(7):e10213. doi: 10.1590/1414-431X2020e10213. eCollection 2021.
5
Long non-coding RNA LINC00473 acts as a microRNA-29a-3p sponge to promote hepatocellular carcinoma development by activating Robo1-dependent PI3K/AKT/mTOR signaling pathway.长链非编码RNA LINC00473作为微小RNA-29a-3p的海绵，通过激活Robo1依赖的PI3K/AKT/mTOR信号通路促进肝细胞癌的发展。
Ther Adv Med Oncol. 2020 Aug 27;12:1758835920937890. doi: 10.1177/1758835920937890. eCollection 2020.
6
Long noncoding TUG1 promotes angiogenesis of HUVECs in PE via regulating the miR-29a-3p/VEGFA and Ang2/Tie2 pathways.长链非编码 TUG1 通过调控 miR-29a-3p/VEGFA 和 Ang2/Tie2 通路促进 PE 中 HUVEC 的血管生成。
Microvasc Res. 2022 Jan;139:104231. doi: 10.1016/j.mvr.2021.104231. Epub 2021 Aug 2.
7
MiR-128-3p suppresses tumor proliferation and metastasis via targeting CDC6 in hepatocellular carcinoma cells.miR-128-3p 通过靶向调控 CDC6 抑制肝癌细胞的增殖和转移
Tissue Cell. 2021 Oct;72:101534. doi: 10.1016/j.tice.2021.101534. Epub 2021 Mar 23.
8
CircPVT1 Promoted the Progression of Breast Cancer by Regulating MiR-29a-3p-Mediated AGR2-HIF-1α Pathway.环状PVT1通过调控miR-29a-3p介导的AGR2-HIF-1α信号通路促进乳腺癌进展
Cancer Manag Res. 2020 Nov 12;12:11477-11490. doi: 10.2147/CMAR.S265579. eCollection 2020.
9
Traditional Chinese Medicine Xiaoai Jiedu Recipe Suppresses the Development of Hepatocellular Carcinoma via Regulating the microRNA-29a/Signal Transducer and Activator of Transcription 3 Axis.中药消癌解毒方通过调控微小RNA-29a/信号转导与转录激活因子3轴抑制肝细胞癌的发展。
Onco Targets Ther. 2020 Jul 29;13:7329-7342. doi: 10.2147/OTT.S248797. eCollection 2020.
10
miR-29a-3p inhibits endometrial cancer cell proliferation, migration and invasion by targeting VEGFA/CD C42/PAK1.miR-29a-3p 通过靶向 VEGFA/CD C42/PAK1 抑制子宫内膜癌细胞增殖、迁移和侵袭。
BMC Cancer. 2021 Jul 21;21(1):843. doi: 10.1186/s12885-021-08506-z.

引用本文的文献

1
miR-29a-3p and TGF-β Axis in Fanconi anemia: mechanisms driving metabolic dysfunction and genome stability.范可尼贫血中的miR-29a-3p与转化生长因子-β轴：驱动代谢功能障碍和基因组稳定性的机制
Cell Mol Life Sci. 2025 Jun 25;82(1):255. doi: 10.1007/s00018-025-05775-w.
2
LncRNA PVT1 Promotes the Progress of Hypertrophic Scar via Regulating the Proliferation and Migration of Myofibroblasts Through Targeting miR-29a-3p/STAT3.长链非编码RNA PVT1通过靶向miR-29a-3p/STAT3调控肌成纤维细胞的增殖和迁移促进增生性瘢痕进展。
Clin Cosmet Investig Dermatol. 2025 Apr 15;18:907-917. doi: 10.2147/CCID.S510079. eCollection 2025.
3

本文引用的文献

1
Loss of miR-107, miR-181c and miR-29a-3p Promote Activation of Notch2 Signaling in Pediatric High-Grade Gliomas (pHGGs).miR-107、miR-181c 和 miR-29a-3p 的缺失促进儿童高级别脑胶质瘤 (pHGG) 中 Notch2 信号的激活。
Int J Mol Sci. 2017 Dec 17;18(12):2742. doi: 10.3390/ijms18122742.
2
Long non-coding RNA H19 regulates E2F1 expression by competitively sponging endogenous miR-29a-3p in clear cell renal cell carcinoma.长链非编码RNA H19通过竞争性结合肾透明细胞癌中的内源性miR-29a-3p来调节E2F1的表达。
Cell Biosci. 2017 Dec 1;7:65. doi: 10.1186/s13578-017-0193-z. eCollection 2017.
3
miR-29a-3p suppresses cell proliferation and migration by downregulating IGF1R in hepatocellular carcinoma.
Bioinformatics analysis and experimental validation of potential targets and pathways in chronic kidney disease associated with renal fibrosis.
慢性肾脏病相关肾纤维化潜在靶点及通路的生物信息学分析与实验验证
J Transl Med. 2025 Apr 2;23(1):387. doi: 10.1186/s12967-024-06058-x.
4
Signaling pathways in liver cancer: pathogenesis and targeted therapy.肝癌信号通路：发病机制与靶向治疗。
Mol Biomed. 2024 May 31;5(1):20. doi: 10.1186/s43556-024-00184-0.
5
TPR is a prognostic biomarker and potential therapeutic target associated with immune infiltration in hepatocellular carcinoma.总外周阻力（TPR）是一种与肝细胞癌免疫浸润相关的预后生物标志物和潜在治疗靶点。
Mol Clin Oncol. 2024 Feb 8;20(4):27. doi: 10.3892/mco.2024.2725. eCollection 2024 Apr.
6
The Role of miRNAs, circRNAs and Their Interactions in Development and Progression of Hepatocellular Carcinoma: An Approach.miRNAs、circRNAs 及其相互作用在肝细胞癌发生发展中的作用：一种研究方法。
Genes (Basel). 2022 Dec 21;14(1):13. doi: 10.3390/genes14010013.
7
Collagen XV mediated the epithelial-mesenchymal transition to inhibit hepatocellular carcinoma metastasis.胶原蛋白XV介导上皮-间质转化以抑制肝细胞癌转移。
J Gastrointest Oncol. 2022 Oct;13(5):2472-2484. doi: 10.21037/jgo-22-299.
8
RNA Therapeutic Options to Manage Aberrant Signaling Pathways in Hepatocellular Carcinoma: Dream or Reality?用于调控肝细胞癌异常信号通路的RNA治疗选择：梦想还是现实？
Front Oncol. 2022 May 4;12:891812. doi: 10.3389/fonc.2022.891812. eCollection 2022.
9
TUG1 promotes the expression of IFITM3 in hepatocellular carcinoma by competitively binding to miR-29a.TUG1通过与miR-29a竞争性结合来促进肝细胞癌中IFITM3的表达。
J Cancer. 2021 Oct 2;12(22):6905-6920. doi: 10.7150/jca.57477. eCollection 2021.
10
Berbamine Inhibits Cell Proliferation and Migration and Induces Cell Death of Lung Cancer Cells via Regulating c-Maf, PI3K/Akt, and MDM2-P53 Pathways.小檗胺通过调节c-Maf、PI3K/Akt和MDM2-P53信号通路抑制肺癌细胞增殖、迁移并诱导其死亡。
Evid Based Complement Alternat Med. 2021 Jul 8;2021:5517143. doi: 10.1155/2021/5517143. eCollection 2021.
微小RNA-29a-3p通过下调肝细胞癌中的胰岛素样生长因子1受体（IGF1R）来抑制细胞增殖和迁移。
Oncotarget. 2017 Sep 23;8(49):86592-86603. doi: 10.18632/oncotarget.21246. eCollection 2017 Oct 17.
4
MiR-192-Mediated Positive Feedback Loop Controls the Robustness of Stress-Induced p53 Oscillations in Breast Cancer Cells.MiR-192介导的正反馈回路控制乳腺癌细胞应激诱导的p53振荡的稳健性。
PLoS Comput Biol. 2015 Dec 7;11(12):e1004653. doi: 10.1371/journal.pcbi.1004653. eCollection 2015 Dec.
5
Inhibiting MDM2-p53 Interaction Suppresses Tumor Growth in Patient-Derived Non-Small Cell Lung Cancer Xenograft Models.抑制 MDM2-p53 相互作用可抑制患者来源的非小细胞肺癌异种移植模型中的肿瘤生长。
J Thorac Oncol. 2015 Aug;10(8):1172-80. doi: 10.1097/JTO.0000000000000584.
6
XPD Functions as a Tumor Suppressor and Dysregulates Autophagy in Cultured HepG2 Cells.XPD在培养的HepG2细胞中作为肿瘤抑制因子发挥作用并失调自噬。
Med Sci Monit. 2015 May 29;21:1562-8. doi: 10.12659/MSM.894303.
7
Reduced miR-29a-3p expression is linked to the cell proliferation and cell migration in gastric cancer.miR-29a-3p表达降低与胃癌中的细胞增殖和细胞迁移有关。
World J Surg Oncol. 2015 Mar 12;13:101. doi: 10.1186/s12957-015-0513-x.
8
MDM2-p53 pathway in hepatocellular carcinoma.肝细胞癌中的MDM2-p53信号通路
Cancer Res. 2014 Dec 15;74(24):7161-7. doi: 10.1158/0008-5472.CAN-14-1446. Epub 2014 Dec 4.
9
MicroRNA-31 is overexpressed in cutaneous squamous cell carcinoma and regulates cell motility and colony formation ability of tumor cells.微小RNA-31在皮肤鳞状细胞癌中过表达，并调节肿瘤细胞的细胞运动性和集落形成能力。
PLoS One. 2014 Jul 28;9(7):e103206. doi: 10.1371/journal.pone.0103206. eCollection 2014.
10
miRNAs affect the development of hepatocellular carcinoma via dysregulation of their biogenesis and expression.微小RNA通过其生物合成和表达的失调影响肝细胞癌的发展。
Cell Commun Signal. 2014 Jul 11;12:45. doi: 10.1186/s12964-014-0045-y.