Salas-Ovilla Roger, Gálvez-López Didiana, Vázquez-Ovando Alfredo, Salvador-Figueroa Miguel, Rosas-Quijano Raymundo
Instituto de Biociencias, Universidad Autónoma de Chiapas, Tapachula, Chiapas, Mexico.
PeerJ. 2019 Jan 3;7:e6102. doi: 10.7717/peerj.6102. eCollection 2019.
Chitin is the second most abundant organic compound in nature and represents a rich carbon and nitrogen source that is primarily transformed by bacterial communities. Bacteria capable of gradually hydrolyzing chitin into N-acetylglucosamine monomers can have applications in the transformation of residues from shrimp and other crustaceans. The objective of the present study was to isolate, characterize and identify microorganisms with high chitinolytic activity. These microorganisms were isolated and characterized based on macro- and microscopic morphological traits. Strains were selected on colloidal chitin agar medium primarily based on a hydrolysis halo larger than 2 mm and a growing phase no longer than 6 days. Secondary selection consisted of semi-quantitative evaluation of chitinolytic activity with a drop dilution assay. From the above, ten strains were selected. Then, strain-specific activity was evaluated. The B4 strain showed the highest specific activity, which was 6,677.07 U/mg protein. Molecular identification indicated that the isolated strains belong to the species .
几丁质是自然界中第二丰富的有机化合物，是一种丰富的碳源和氮源，主要由细菌群落进行转化。能够将几丁质逐步水解为N-乙酰葡糖胺单体的细菌可应用于虾及其他甲壳类动物残渣的转化。本研究的目的是分离、表征和鉴定具有高几丁质分解活性的微生物。这些微生物基于宏观和微观形态特征进行分离和表征。主要在胶体几丁质琼脂培养基上根据水解晕圈大于2毫米且生长阶段不超过6天来选择菌株。二次筛选包括用滴度稀释法对几丁质分解活性进行半定量评估。据此，选择了10个菌株。然后，评估菌株的比活性。B4菌株显示出最高的比活性，为6677.07 U/mg蛋白质。分子鉴定表明分离出的菌株属于 物种。
