Faculty of Information Technology and Communication Sciences, Tampere University, Tampere, Finland. BioMediTech Institute and Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland. Author to whom any correspondence should be addressed.
Phys Med Biol. 2019 Feb 18;64(4):045017. doi: 10.1088/1361-6560/aafd63.
Solving the fluorophore distribution in a tomographic setting has been difficult because of the lack of physically meaningful and computationally applicable propagation models. This study concentrates on the direct modelling of fluorescence signals in optical projection tomography (OPT), and on the corresponding inverse problem. The reconstruction problem is solved using emission projections corresponding to a series of rotational imaging positions of the sample. Similarly to the bright field OPT bearing resemblance with the transmission x-ray computed tomography, the fluorescent mode OPT is analogous to x-ray fluorescence tomography (XFCT). As an improved direct model for the fluorescent OPT, we derive a weighted Radon transform based on the XFCT literature. Moreover, we propose a simple and fast iteration scheme for the slice-wise reconstruction of the sample. The developed methods are applied in both numerical experiments and inversion of fluorescent OPT data from a zebrafish embryo. The results demonstrate the importance of propagation modelling and our analysis provides a flexible modelling framework for fluorescent OPT that can easily be modified to adapt to different imaging setups.
由于缺乏具有物理意义和计算适用性的传播模型，解决断层成像中的荧光团分布问题一直具有挑战性。本研究专注于光学投影断层成像（OPT）中荧光信号的直接建模，以及相应的逆问题。重建问题通过使用与样品旋转成像位置系列对应的发射投影来解决。类似于具有类似于透射 X 射线计算机断层扫描的亮场 OPT，荧光模式 OPT 类似于 X 射线荧光断层扫描（XFCT）。作为荧光 OPT 的改进直接模型，我们根据 XFCT 文献推导出基于加权 Radon 变换的模型。此外，我们还为样本的切片式重建提出了一种简单快速的迭代方案。所开发的方法已应用于荧光 OPT 的数值实验和反转。结果表明了传播建模的重要性，并且我们的分析为荧光 OPT 提供了一个灵活的建模框架，可以轻松修改以适应不同的成像设置。
