Duan Y L, Zhu Y, Xu B P, Li C C, Chen A H, Deng L, Bao Y X, Cao L, Sun Y, Ning L M, Fu Z, Liu C Y, Yin J, Shen K L, Zhou Y L, Xie Z D
Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, National Key Discipline of Pediatrics (Capital Medical University), Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China.
the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, China.
Zhonghua Er Ke Za Zhi. 2019 Jan 2;57(1):27-32. doi: 10.3760/cma.j.issn.0578-1310.2019.01.008.
To investigate the predominant genotypes and epidemiological characteristics of human adenovirus (HAdV) in pediatric community-acquired pneumonia (CAP) in China. This was a repeated cross sectional study. Between November 2014 and November 2016, nasopharyngeal aspirates (NPAs) or throat swabs from each hospitalized pediatric patients diagnosed as CAP in 12 hospitals in Northern and Southern China were collected. Respiratory specimens were screened for 18 respiratory viruses including HAdV by using Luminex xTAG RVP Fast V2 multiplex Assay. Typing of HAdV and analysis for the epidemiological characteristic of HAdV were performed. (1) A total of 2 723 hospitalized pediatric patients with CAP were enrolled in this study and 156 (5.7%, 156/2 723) respiratory specimens were positive for HAdV, and 74 (6.6%, 74/1 128) and 82 (5.1%, 82/1 595) were in Northern and Southern China, respectively. There was no significant difference in the positive detection rate between the Northern and Southern China. (2) In Northern China, the HAdV positive rate of children at the age of <6 months, 6 months-<1 years, 1-<3 years, 3-<5 years and ≥5 years was 5.9%(6/101), 6.7%(7/104), 10.3%(34/331), 4.1%(11/266) and 4.9%(16/326), respectively, and the incidence of HAdV infection peaked in children aged 1-3 years (χ(2)=11.511, 0.021). While in Southern China the HAdV positive rate of children at the age of <6 months, 6 months-<1 years, 1-<3 years, 3-<5 years and ≥5 years was 2.2% (7/312), 4.6% (12/259), 6.3% (31/494), 7.3% (18/245) and 4.9%(14/285), respectively. There was no significant difference in the positive detection rate among age groups. (3) In 2015, the highest detection rate of HAdV in northern China was 12.5% (25/200) in winter, and in Southern China was 6.7% (35/525) in spring and 5.3% (19/357) in summer. (4) In 108 cases of HAdV positive specimens typing was done and 80 in cases classification was successfully performed.Totally 7 genotypes of HAdV, including HAdV-3 (32), HAdV-7 (9), HAdV-1 (12), HAdV-2 (15), HAdV-5 (10), HAdV-6 (1) and HAdV-4 (1), were detected. The predominant HAdV genotypes were HAdV-3 (30.8%, 8/26) and HAdV-7 (26.9%, 7/26) in Northern China, while HAdV-3 (44.4%, 24/54) and HAdV-2 (22.2%, 12/54) were the most prevalent genotypes in Southern China. HAdV is an important viral pathogen in pediatric CAP. The predominant HAdV genotypes and peak seasons of HAdV infections were different between Northern and Southern China. The predominant HAdV genotypes were HAdV-3 and HAdV-7 in Northern China, while HAdV-3 and HAdV-2 in Southern China. The peak season of HAdV infections was winter in Northern China. However, HAdV infections are more common in spring and summer in Southern China.
为研究中国儿童社区获得性肺炎（CAP）中人类腺病毒（HAdV）的主要基因型及流行病学特征。这是一项重复横断面研究。2014年11月至2016年11月，收集了中国北方和南方12家医院中每名诊断为CAP的住院儿童患者的鼻咽抽吸物（NPA）或咽拭子。采用Luminex xTAG RVP Fast V2多重检测法对呼吸道标本进行18种呼吸道病毒筛查，包括HAdV。对HAdV进行分型并分析其流行病学特征。（1）本研究共纳入2723例住院CAP儿童患者，156份（5.7%，156/2723）呼吸道标本HAdV检测呈阳性，其中中国北方74份（6.6%，74/1128），南方82份（5.1%，82/1595）。中国北方和南方的阳性检出率无显著差异。（2）在中国北方，<6个月、6个月至<1岁、1至<3岁、3至<5岁及≥5岁儿童的HAdV阳性率分别为5.9%（6/101）、6.7%（7/104）、10.3%（34/331）、4.1%（11/266）和4.9%（16/326），HAdV感染发病率在1至3岁儿童中达到峰值（χ(2)=11.511，P=0.021）。而在中国南方，<6个月、6个月至<1岁、1至<3岁、3至<5岁及≥5岁儿童的HAdV阳性率分别为2.2%（7/312）、4.6%（12/259）、6.3%（31/494）、7.3%（18/245）和4.9%（14/285）。各年龄组的阳性检出率无显著差异。（3）2015年，中国北方HAdV最高检出率在冬季为12.5%（25/200），中国南方在春季为6.7%（35/525），夏季为5.3%（19/357）。（4）对108份HAdV阳性标本进行了分型，80份成功进行了分类。共检测到7种HAdV基因型，包括HAdV-3（32例）、HAdV-7（9例）、HAdV-1（12例）、HAdV-2（15例）、HAdV-5（10例）、HAdV-6（1例）和HAdV-4（1例）。中国北方主要的HAdV基因型为HAdV-3（30.8%，8/26）和HAdV-7（26.9%，7/26），而中国南方最常见的基因型为HAdV-3（44.4%，24/54）和HAdV-2（22.2%，12/54）。HAdV是儿童CAP中的重要病毒病原体。中国北方和南方HAdV感染的主要基因型和高峰季节不同。中国北方主要的HAdV基因型为HAdV-3和HAdV-7，而中国南方为HAdV-3和HAdV-2。中国北方HAdV感染的高峰季节是冬季。然而，中国南方HAdV感染在春季和夏季更为常见。
