中国北京 2017-2018 年住院呼吸道感染患儿中的人腺病毒。
Human adenovirus among hospitalized children with respiratory tract infections in Beijing, China, 2017-2018.
机构信息
NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, 100 Ying-Xin St., Xi-Cheng District, Beijing, 100052, China.
Department of Pediatrics, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China.
出版信息
Virol J. 2019 Jun 13;16(1):78. doi: 10.1186/s12985-019-1185-x.
BACKGROUND
Human adenoviruses (HAdVs) cause a wide range of diseases. However, the genotype diversity and epidemiological information relating to HAdVs among hospitalized children with respiratory tract infections (RTIs) is limited. Here, we describe the epidemiology and genotype distribution of HAdVs associated with RTIs in Beijing, China.
METHODS
Nasopharyngeal aspirates (NPA) were collected from hospitalized children with RTIs from April 2017 to March 2018. HAdVs were detected by a TaqMan-based quantitative real-time polymerase chain reaction (qPCR) assay, and the hexon gene was used for phylogenetic analysis. Epidemiological data were analyzed using statistical product and service solutions (SPSS) 21.0 software.
RESULTS
HAdV was detected in 72 (5.64%) of the 1276 NPA specimens, with most (86.11%, 62/72) HAdV-positives cases detected among children < 6 years of age. HAdV-B3 (56.06%, 37/66) and HAdV-C2 (19.70%, 13/66) were the most frequent. Of the 72 HAdV-infected cases, 27 (37.50%) were co-infected with other respiratory viruses, most commonly parainfluenza virus (12.50%, 9/72) and rhinovirus (9.72%, 7/72). The log number of viral load ranged from 3.30 to 9.14 copies per mL of NPA, with no significant difference between the HAdV mono- and co-infection groups. The main clinical symptoms in the HAdV-infected patients were fever and cough, and 62 (86.11%, 62/72) were diagnosed with pneumonia. Additionally, HAdVs were detected throughout the year with a higher prevalence in summer.
CONCLUSIONS
HAdV prevalence is related to age and season. HAdV-B and HAdV-C circulated simultaneously among the hospitalized children with RTIs in Beijing, and HAdV-B type 3 and HAdV-C type 2 were the most frequent.
背景
人类腺病毒（HAdVs）可引起多种疾病。然而，关于住院呼吸道感染（RTI）患儿中 HAdVs 的基因型多样性和流行病学信息有限。在这里，我们描述了中国北京与 RTI 相关的 HAdVs 的流行病学和基因型分布。
方法
采集 2017 年 4 月至 2018 年 3 月住院 RTI 患儿的鼻咽抽吸物（NPA）。采用 TaqMan 实时定量聚合酶链反应（qPCR）检测 HAdV，并用 hexon 基因进行系统进化分析。采用统计产品与服务解决方案（SPSS）21.0 软件分析流行病学数据。
结果
在 1276 份 NPA 标本中，检测到 72 份（5.64%）HAdV，86.11%（62/72）的 HAdV 阳性病例出现在<6 岁儿童中。HAdV-B3（56.06%，37/66）和 HAdV-C2（19.70%，13/66）是最常见的。在 72 例 HAdV 感染病例中，27 例（37.50%）与其他呼吸道病毒同时感染，最常见的是副流感病毒（12.50%，9/72）和鼻病毒（9.72%，7/72）。病毒载量的对数范围从 3.30 到 9.14 拷贝/ml 的 NPA，HAdV 单感染组和混合感染组之间无显著差异。HAdV 感染患者的主要临床症状为发热和咳嗽，62 例（86.11%，62/72）被诊断为肺炎。此外，HAdV 全年均可检测到，夏季流行率较高。
结论
HAdV 的流行与年龄和季节有关。在北京住院 RTI 患儿中，HAdV-B 和 HAdV-C 同时流行，HAdV-B 型 3 和 HAdV-C 型 2 最为常见。
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