Yan Xiao-Rui, Tuo Li
Research Center for Medicine and Biology, Zunyi Medical University, Zunyi, 563006, People's Republic of China.
Zunyi Engineering Research Center of Physical Testing and Chemical Analysis, Zunyi Medical University, Zunyi, 563006, People's Republic of China.
Antonie Van Leeuwenhoek. 2019 Jun;112(6):919-925. doi: 10.1007/s10482-019-01225-3. Epub 2019 Jan 10.
A Gram-stain negative, aerobic, coccus-shaped bacterium, designated strain M2BS9Y-3-1, was isolated from a surface-sterilized leaf of Rhizophora stylosa. It grew in the pH range 5.0-9.0 (optimum, 7.0) and at temperatures between 20 and 37 °C (optimum, 30 °C). Growth was possible at concentrations of 1-10% (w/v) NaCl (optimum, 2%). The phylogenetic trees based on 16S rRNA gene sequences indicated that strain M2BS9Y-3-1 clustered together with Notoacmeibacter marinus XMTR2A4 (98.44% similarity) and separated from other species of the family Notoacmeibacteraceae. The average nucleotide identity and in silico DNA-DNA hybridization values between strain M2BS9Y-3-1 and N. marinus XMTR2A4 were 76.8%, 19.8%, respectively. The G+C content of the genomic DNA was 60.0 mol%. The cell wall peptidoglycan contained meso-diaminobutyric acid and ubiquinone Q-10 was the respiratory lipoquinone. The major fatty acid was Cω7c. The lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, an unidentified aminophospholipid and three unidentified lipids. The results of taxonomic analysis suggested that strain M2BS9Y-3-1 represents a novel species of the genus Notoacmeibacter for which the name Notoacmeibacter ruber sp. nov. is proposed. The type strain is M2BS9Y-3-1 (KCTC 62838 = CGMCC 1.13746).
从经表面消毒的红海榄叶片中分离出一株革兰氏染色阴性、需氧、球菌状细菌，命名为菌株M2BS9Y-3-1。它在pH值5.0 - 9.0（最适pH值7.0）范围内以及20至37°C（最适温度30°C）之间生长。在1 - 10%（w/v）NaCl浓度下（最适浓度2%）能够生长。基于16S rRNA基因序列构建的系统发育树表明，菌株M2BS9Y-3-1与海栖诺阿克米杆菌XMTR2A4聚在一起（相似度为98.44%），并与诺阿克米杆菌科的其他物种分开。菌株M2BS9Y-3-1与海栖诺阿克米杆菌XMTR2A4之间的平均核苷酸同一性和电子计算机DNA - DNA杂交值分别为76.8%、19.8%。基因组DNA的G + C含量为60.0 mol%。细胞壁肽聚糖含有内消旋二氨基丁酸，呼吸性脂醌为泛醌Q - 10。主要脂肪酸为Cω7c。脂质包括双磷脂酰甘油、磷脂酰甘油、磷脂酰胆碱、磷脂酰单甲基乙醇胺、磷脂酰乙醇胺、一种未鉴定的氨基磷脂和三种未鉴定的脂质。分类学分析结果表明，菌株M2BS9Y-3-1代表诺阿克米杆菌属的一个新物种，为此提出新名称红色诺阿克米杆菌（Notoacmeibacter ruber sp. nov.）。模式菌株为M2BS9Y-3-1（KCTC 62838 = CGMCC 1.13746）。
