Life Sciences Institute, Zunyi Medical University, Zunyi, 563006, People's Republic of China.
Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, National Key Discipline of Pediatrics (Capital Medical University), Beijing Pediatric Research Institute, Beijing Children's Hospital, National Center for Children's Health, Capital Medical University, Beijing, 100045, People's Republic of China.
Antonie Van Leeuwenhoek. 2021 Oct;114(10):1633-1645. doi: 10.1007/s10482-021-01629-0. Epub 2021 Jul 31.
A Gram-stain negative, non-motile, brilliant yellow and non-spore forming, coccoid- or short rod-shaped bacterium, designated strain KSK16Y-1, was isolated from surface-sterilised leaf of Rhizophora stylosa collected from Shankou Mangrove Nature Reserve, Guangxi Zhuang Autonomous Region, China. Genome of strain KSK16Y-1 is 4.93 Mb with 68.1% DNA G + C content and encoded 4359 predicted proteins, 4 rRNAs, 45 tRNAs and 4 ncRNA. Comparative 16S rRNA gene sequence analysis showed that the strain KSK16Y-1 has 98.1%, 97.9% and 96.9% 16S rRNA gene similarities with Jiella aquimaris JCM 30119, J. endophytica CBS5Q-3 and J. pacifica 40Bstr34, respectively. Whole-genome comparisons between strain KSK16Y-1 and J. aquimaris 22II-16-19i, J. endophytica CBS5Q-3, J. pacifica 40Bstr34, using average nucleotide identity (ANI) values (< 82.0%) and digital DNA-DNA hybridization (dDDH) values (< 25.1%), confirmed low genome relatedness. Strain KSK16Y-1 grew at 20-30 °C (optimum, 30 °C), pH 6.0-11.0 (optimum, pH 6.0-7.0) and with 0-10% (w/v) NaCl (optimum, 0-2%). Cell wall contained meso-diaminopimelic acid and the major fatty acid was Cω7c. The polar lipid profile consists of phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, one unknown phospholipid, one unknown aminolipid, one unknown aminophospholipid and four unidentified lipids. The predominant respiratory quinone is ubiquinone-10 (Q-10). The polyphasic characterization indicated that strain KSK16Y-1 represents a novel Jiella species. The name Jiella mangrovi sp. nov., type strain KSK16Y-1 (= CGMCC 1.18745 = JCM 34332) is proposed.
一株革兰氏阴性、非运动、亮黄色、不产芽孢、球形或短杆形细菌，被命名为KSK16Y-1 菌株，从中国广西壮族自治区山口红树林自然保护区表面消毒的红树属Stylosa 叶片中分离得到。KSK16Y-1 菌株的基因组为 4.93Mb，GC 含量为 68.1%，编码 4359 个预测蛋白、4 个 rRNA、45 个 tRNA 和 4 个 ncRNA。比较 16S rRNA 基因序列分析表明，该菌株与 Jiella aquimaris JCM 30119、J. endophytica CBS5Q-3 和 J. pacifica 40Bstr34 的 16S rRNA 基因相似度分别为 98.1%、97.9%和 96.9%。与 Jiella aquimaris 22II-16-19i、J. endophytica CBS5Q-3、J. pacifica 40Bstr34 的全基因组比较，使用平均核苷酸同一性（ANI）值（<82.0%）和数字 DNA-DNA 杂交（dDDH）值（<25.1%），证实了基因组的低相关性。KSK16Y-1 菌株在 20-30°C（最佳温度 30°C）、pH 6.0-11.0（最佳 pH 6.0-7.0）和 0-10%（w/v）NaCl（最佳 0-2%）条件下生长。细胞壁含有 meso-二氨基庚二酸，主要脂肪酸为 Cω7c。极性脂谱由磷脂酰胆碱、磷脂酰甘油、双磷脂酰甘油、磷脂酰乙醇胺、磷脂单甲乙醇胺、一种未知磷脂、一种未知胺脂、一种未知胺磷脂和四种未知脂组成。主要呼吸醌为泛醌-10（Q-10）。多相特征表明，KSK16Y-1 菌株代表一种新的 Jiella 种。建议将该菌株命名为 Jiella mangrovi sp. nov.，其模式菌株为 KSK16Y-1（=CGMCC 1.18745=JCM 34332）。
