Dragúňová Jana, Kabát Peter, Cucorová Valéria, Hajská Marianna, Koller Ján
Department of Burns and Reconstructive Surgery, Faculty of Medicine, Comenius University, Bratislava, Slovakia.
Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, Mlynská Dolina, Ilkovičova 6, 842 15, Bratislava 4, Slovakia.
Cell Tissue Bank. 2019 Mar;20(1):35-48. doi: 10.1007/s10561-018-09747-2. Epub 2019 Jan 10.
Amniotic membrane is a biological material widely used in plastic and reconstructive surgery and in ophthalmology. Due to its excellent biocompatibility and strength we tried to use it as a scaffold for the in vitro cultivation of different cell types, especially keratinocytes and limbal stem cells. It was possible to cultivate limbal stem cells and keratinocytes without using 3T3 mouse fibroblast feeder cells on deep frozen amniotic membranes. The amniotic membrane can also be used as a carrier for suspensions of different types of cells, allowing a substantial reduction of the cultivation time needed to prepare cell cultures for clinical application to burn patients. Our results show that the amniotic membrane seems not only to be an excellent carrier for human keratinocytes and corneal limbal stem cells, but also for other cell types, including dermal fibroblasts, adipose tissue-derived mesenchymal stem cells and chondrocytes.
羊膜是一种广泛应用于整形和重建外科以及眼科的生物材料。由于其优异的生物相容性和强度，我们尝试将其用作不同细胞类型体外培养的支架，尤其是角质形成细胞和角膜缘干细胞。在深度冷冻的羊膜上不使用3T3小鼠成纤维细胞饲养层细胞就可以培养角膜缘干细胞和角质形成细胞。羊膜还可以用作不同类型细胞悬液的载体，从而大幅缩短为烧伤患者制备临床应用细胞培养物所需的培养时间。我们的结果表明，羊膜似乎不仅是人类角质形成细胞和角膜缘干细胞的优良载体，也是其他细胞类型的优良载体，包括真皮成纤维细胞、脂肪组织来源的间充质干细胞和软骨细胞。
