Dr Stanislaw Sakiel's Center for Burn Treatment, Jana Pawła II 2, 41-100 Siemianowice Śląskie, Poland; Silesian Higher Medical School in Katowice, Mickiewicza 29, 40-085 Katowice, Poland.
Dr Stanislaw Sakiel's Center for Burn Treatment, Jana Pawła II 2, 41-100 Siemianowice Śląskie, Poland; Silesian Higher Medical School in Katowice, Mickiewicza 29, 40-085 Katowice, Poland.
Eur J Pharmacol. 2019 Jul 5;854:167-178. doi: 10.1016/j.ejphar.2019.02.043. Epub 2019 Feb 28.
An alternative to cultured skin cell grafts usage in burn treatment is the graft of allogenic stem cells. We verified whether amniotic stem cells are better than the present therapeutic standard: grafts of autologous keratinocytes and fibroblasts along with autologous adipose-derived stem cells, and whether amniotic stem cells can support the growth of autologous keratinocytes and fibroblasts in the culture. The study was performed on the material from 18 amnia. Skin cells were obtained from 3 patients. In order to assess the influence of stem cells on keratinocytes and fibroblasts, the following experiments were performed: impact on viability and cell cycle, wound healing capability, angiogenesis capability, influence on the proliferation speed and capability to differentiate into skin cells. We demonstrated that human amniotic membrane-derived mesenchymal stem cells (hAMMSCs) share amniotic proteins with skin cells. Amniotic stem cells may replace skin fibroblasts in grafts due to the close similarity in their surface antigens, with significantly larger proliferative potential and ability to stimulate wound healing. It was shown that adding amniotic cells to both keratinocytes and fibroblast cultures accelerates directional migration by ≥ 40%. We confirmed in this study the influence of amniotic cells on the proliferation and cell cycle of fibroblasts and keratinocytes. Amniotic stem cells can be successfully used not only as a first choice graft but also to replace 3T3 line cells, supporting the proliferation of the cells during the culturing, as well as a supplementary graft supporting an autologous graft of keratinocytes and fibroblasts.
在烧伤治疗中，替代培养的皮肤细胞移植物的方法是同种异体干细胞移植。我们验证了羊膜干细胞是否优于目前的治疗标准：自体角质形成细胞和成纤维细胞与自体脂肪来源的干细胞移植，以及羊膜干细胞是否能够支持自体角质形成细胞和成纤维细胞在培养中的生长。该研究使用了 18 份羊水标本。从 3 名患者中获得了皮肤细胞。为了评估干细胞对角质形成细胞和成纤维细胞的影响，进行了以下实验：对细胞活力和细胞周期、伤口愈合能力、血管生成能力、增殖速度和向皮肤细胞分化能力的影响。我们证明了人羊膜间充质干细胞（hAMMSCs）与人皮肤细胞共享羊膜蛋白。由于表面抗原非常相似，羊膜干细胞可能替代皮肤成纤维细胞用于移植物，其具有显著更大的增殖潜力和刺激伤口愈合的能力。结果表明，将羊膜细胞添加到角质形成细胞和成纤维细胞培养物中可使定向迁移速度提高≥40%。本研究证实了羊膜细胞对成纤维细胞和角质形成细胞增殖和细胞周期的影响。羊膜干细胞不仅可以成功用作首选移植物，还可以替代 3T3 细胞系，在培养过程中支持细胞增殖，并作为支持自体角质形成细胞和成纤维细胞移植物的补充移植物。
