Tropical Medicine Institute "Pedro Kourí" (IPK), Center for Research, Diagnosis and Reference, La Habana, Cuba.
Tropical Medicine Institute "Pedro Kourí" (IPK), Center for Research, Diagnosis and Reference, La Habana, Cuba.
Int J Infect Dis. 2019 Mar;80:115-117. doi: 10.1016/j.ijid.2018.12.018. Epub 2019 Jan 10.
Mycoplasma pneumoniae is a common cause of community-acquired pneumonia in humans. Treatment of infections can be complicated by the occurrence of macrolide resistant strains. The study was conducted to evaluate the presence of resistant strains in Cuba and to determine the corresponding genotypes.
DNA of M. pneumoniae isolates and positive respiratory tract specimens collected in the years 2012 and 2017 were tested for resistance-associated mutations of 23S rRNA. In addition, strain types (P1 and MLVA) were determined.
Macrolide resistance mutations were confirmed in 5 out of 27 strains (18.5%). Whereas both P1 subtypes 1 and 2 as well variants V2a and V2c were identified, only two MLVA types (4/5/7/2 and 3/5/6/2) could be found.
During both sampling years, circulation of macrolide resistant strains was demonstrated. No association of resistance with a particular P1/MLVA type was found. Future longitudinal sampling to monitor prevalence of macrolide resistance of M. pneumoniae is recommended to verify the resistance pattern of this important pathogen of human respiratory tract infections.
肺炎支原体是人社区获得性肺炎的常见病因。感染的治疗可能因大环内酯类耐药株的出现而变得复杂。本研究旨在评估古巴耐药株的存在情况，并确定相应的基因型。
对 2012 年和 2017 年采集的肺炎支原体分离株和阳性呼吸道标本的 DNA 进行 23S rRNA 耐药相关突变检测。此外，还确定了菌株类型（P1 和 MLVA）。
在 27 株菌中，有 5 株（18.5%）证实存在大环内酯类耐药突变。虽然鉴定出了 P1 亚型 1 和 2 以及变体 V2a 和 V2c，但仅发现了两种 MLVA 类型（4/5/7/2 和 3/5/6/2）。
在两个采样年份都证明了大环内酯类耐药株的传播。未发现耐药性与特定的 P1/MLVA 类型有关。建议未来进行纵向采样，以监测肺炎支原体大环内酯类耐药的流行情况，以验证这种重要的人类呼吸道感染病原体的耐药模式。
