从水培甜叶菊根中提取菊粉型果聚糖及其在食品应用中的特性研究
Extraction and Characterization of Inulin-Like Fructans from Hydroponically Grown Stevia rebaudiana Roots for Food Applications.
作者信息
Lima Hevelyn Regina da Silva, Santos Gabriela Lafayne Okonski Dos, Ribeiro Susana Tavares Cotrim, Gonçalves Maria Amélia, Savam Aline, Gonçalves Regina Aparecida Correia, de Oliveira Arildo José Braz
机构信息
Departamento de Biotecnologia, Genética e Biologia Celular, Programa de Pós-Graduação em Biotecnologia Ambiental, Universidade Estadual de Maringá, Av. Colombo 5790, Maringá, Paraná, 87020-900, Brazil.
Departamento de Farmácia, Programa de Pós-Graduação em Ciências Farmacêuticas, Laboratório de Biotecnologia de Produtos Naturais e Sintéticos (LABIPROS), Universidade Estadual de Maringá, Av. Colombo 5790, Maringá, Paraná, 87020-900, Brazil.
出版信息
Plant Foods Hum Nutr. 2025 Jan 14;80(1):36. doi: 10.1007/s11130-024-01266-8.
Stevia rebaudiana is a plant native to South America known for producing steviol glycosides and fructans used in low-calorie and functional foods. This study aimed to cultivate and isolate inulin from hydroponically grown S. rebaudiana roots. Seedlings were grown hydroponically for 28 days; then, roots were dried and extracted at 121 °C for 20 min. Extracts were centrifuged and lyophilized, yielding supernatant (SSR-H) and precipitate (PSR-H) fractions. Fructan yields were 21.34% (SSR-H) and 2.91% (PSR-H). HPLC, FTIR, and NMR analyses confirmed inulin-like fructans with polymerization degrees of 16 and 31, respectively. The characteristic peaks of a fructan-like-inulin were observed in the FTIR, ¹H, and ¹³C NMR spectra. These results demonstrate effective inulin extraction from hydroponically grown S. rebaudiana roots, highlighting its potential applications in the food industry.
甜叶菊是一种原产于南美洲的植物，以生产用于低热量和功能性食品的甜菊糖苷和果聚糖而闻名。本研究旨在从水培甜叶菊的根中培养并分离菊粉。幼苗水培生长28天；然后，将根干燥并在121°C下提取20分钟。提取物经离心和冻干，得到上清液（SSR-H）和沉淀（PSR-H）部分。果聚糖产量分别为21.34%（SSR-H）和2.91%（PSR-H）。HPLC、FTIR和NMR分析证实了聚合度分别为16和31的菊粉样果聚糖。在FTIR、¹H和¹³C NMR光谱中观察到了果聚糖样菊粉的特征峰。这些结果表明从水培甜叶菊根中有效提取菊粉，突出了其在食品工业中的潜在应用。
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