Can Adem, Dao David T, Arad Michal, Terrillion Chantelle E, Piantadosi Sean C, Gould Todd D
Department of Psychiatry, University of Maryland School of Medicine, USA.
J Vis Exp. 2012 Jan 29(59):e3638. doi: 10.3791/3638.
The forced swim test is a rodent behavioral test used for evaluation of antidepressant drugs, antidepressant efficacy of new compounds, and experimental manipulations that are aimed at rendering or preventing depressive-like states. Mice are placed in an inescapable transparent tank that is filled with water and their escape related mobility behavior is measured. The forced swim test is straightforward to conduct reliably and it requires minimal specialized equipment. Successful implementation of the forced swim test requires adherence to certain procedural details and minimization of unwarranted stress to the mice. In the protocol description and the accompanying video, we explain how to conduct the mouse version of this test with emphasis on potential pitfalls that may be detrimental to interpretation of results and how to avoid them. Additionally, we explain how the behaviors manifested in the test are assessed.
强迫游泳试验是一种啮齿动物行为试验，用于评估抗抑郁药物、新化合物的抗抑郁效果以及旨在产生或预防类抑郁状态的实验操作。将小鼠置于一个装满水的不可逃脱的透明水箱中，测量其与逃脱相关的活动行为。强迫游泳试验操作简单且可靠，所需的专业设备最少。成功实施强迫游泳试验需要遵循某些程序细节，并尽量减少对小鼠不必要的应激。在方案描述和随附视频中，我们将解释如何进行该试验的小鼠版本，重点介绍可能对结果解释不利的潜在陷阱以及如何避免这些陷阱。此外，我们还将解释如何评估试验中表现出的行为。
