Fujian Biological Materials Engineering and Technology Center of Stomatology, School and Hospital of Stomatology, Fujian Medical University, 246 Yangqiao Zhong Rd, Fuzhou, 350002, Fujian, China.
J Mol Histol. 2019 Apr;50(2):105-117. doi: 10.1007/s10735-019-09810-6. Epub 2019 Jan 11.
Craniofacial autologous bone grafts offer superior outcomes to long bone grafts in the reconstruction of maxillofacial bone defects, but the mechanism responsible for this superiority has not yet been illustrated clearly. Osteoblasts play vital roles in bone development and regeneration. However, presently, only a few studies have compared the osteogenic ability of osteoblasts from craniofacial and long bones, and the results are contradictory. Additionally, the angiogenic characteristics of osteoblasts from these different bones remain unknown. We obtained osteoblasts from the rat mandible (MOBs) and femur (FOBs) to investigate their proliferative capacity and osteogenic potential, and using a co-culture system with human umbilical vein endothelial cells (HUVECs), we explored their angiogenic capabilities in vitro. FOBs exhibited higher alkaline phosphatase activity and increased matrix mineralization and expressed more osteogenic related marker genes, while MOBs proliferated at the highest rate and showed elevated expression of angiogenesis-related factors. Conditioned media from MOBs enhanced the expression of angiogenesis-related factors in HUVECs. Furthermore, the conditioned media generated from MOBs showed stronger promotion of proliferation, migration, and tube-like structure formation in HUVECs, suggesting that MOBs had a stronger pro-angiogenic effect on HUVECs than FOBs. Taken together, these results indicate that osteoblasts possess skeletal site-specific differences in osteogenic and angiogenic capabilities, and this might lead to a better understanding of the molecular impact of bone cells from different bone entities on maxillofacial bone reconstructions.
颅颌面自体骨移植在重建颌面部骨缺损方面优于长骨移植，但负责这种优势的机制尚未得到明确阐述。成骨细胞在骨发育和再生中起着至关重要的作用。然而，目前仅有少数研究比较了颅颌面骨和长骨来源的成骨细胞的成骨能力，且结果相互矛盾。此外，这些不同来源的成骨细胞的血管生成特性也尚不清楚。我们从大鼠下颌骨（MOBs）和股骨（FOBs）中获取成骨细胞，以研究其增殖能力和成骨潜能，并通过与人脐静脉内皮细胞（HUVECs）的共培养系统，探讨其体外血管生成能力。FOBs 表现出更高的碱性磷酸酶活性和增加的基质矿化，并表达更多的成骨相关标记基因，而 MOBs 的增殖速度最快，且血管生成相关因子的表达水平升高。MOBs 的条件培养基增强了 HUVECs 中血管生成相关因子的表达。此外，MOBs 产生的条件培养基对 HUVECs 的增殖、迁移和管状结构形成的促进作用更强，表明 MOBs 对 HUVECs 的促血管生成作用强于 FOBs。综上所述，这些结果表明成骨细胞在成骨和血管生成能力方面具有骨骼部位特异性差异，这可能有助于更好地理解来自不同骨实体的骨细胞对颌面部骨重建的分子影响。
