Xu Xiongcheng, Xiao Long, Xu Yanmei, Zhuo Jin, Yang Xue, Li Li, Xiao Nianqi, Tao Jing, Zhong Quan, Li Yanfen, Chen Yuling, Du Zhibin, Luo Kai
Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key Laboratory of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350002, China.
Institute of Stomatology & Laboratory of Oral Tissue Engineering, School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350002, China.
Regen Biomater. 2021 Nov 12;8(6):rbab061. doi: 10.1093/rb/rbab061. eCollection 2021 Dec.
Critical oral-maxillofacial bone defects, damaged by trauma and tumors, not only affect the physiological functions and mental health of patients but are also highly challenging to reconstruct. Personalized biomaterials customized by 3D printing technology have the potential to match oral-maxillofacial bone repair and regeneration requirements. Laponite (LAP) nanosilicates have been added to biomaterials to achieve biofunctional modification owing to their excellent biocompatibility and bioactivity. Herein, porous nanosilicate-functionalized polycaprolactone (PCL/LAP) was fabricated by 3D printing technology, and its bioactivities in bone regeneration were investigated and . experiments demonstrated that PCL/LAP exhibited good cytocompatibility and enhanced the viability of bone marrow mesenchymal stem cells (BMSCs). PCL/LAP functioned to stimulate osteogenic differentiation of BMSCs at the mRNA and protein levels and elevated angiogenic gene expression and cytokine secretion. Moreover, BMSCs cultured on PCL/LAP promoted the angiogenesis potential of endothelial cells by angiogenic cytokine secretion. Then, PCL/LAP scaffolds were implanted into the calvarial defect model. Toxicological safety of PCL/LAP was confirmed, and significant enhancement of vascularized bone formation was observed. Taken together, 3D-printed PCL/LAP scaffolds with brilliant osteogenesis to enhance bone regeneration could be envisaged as an outstanding bone substitute for a promising change in oral-maxillofacial bone defect reconstruction.
由创伤和肿瘤导致的严重口腔颌面骨缺损不仅会影响患者的生理功能和心理健康，而且对修复来说也极具挑战性。通过3D打印技术定制的个性化生物材料有潜力满足口腔颌面骨修复和再生的需求。由于具有优异的生物相容性和生物活性，锂皂石（LAP）纳米硅酸盐已被添加到生物材料中以实现生物功能改性。在此，通过3D打印技术制备了多孔纳米硅酸盐功能化聚己内酯（PCL/LAP），并研究了其在骨再生中的生物活性。实验表明，PCL/LAP表现出良好的细胞相容性，并提高了骨髓间充质干细胞（BMSC）的活力。PCL/LAP在mRNA和蛋白质水平上刺激BMSC的成骨分化，提高血管生成基因表达和细胞因子分泌。此外，在PCL/LAP上培养的BMSC通过分泌血管生成细胞因子促进内皮细胞的血管生成潜力。然后，将PCL/LAP支架植入颅骨缺损模型中。证实了PCL/LAP的毒理学安全性，并观察到血管化骨形成显著增强。综上所述，具有出色成骨能力以促进骨再生的3D打印PCL/LAP支架有望成为口腔颌面骨缺损重建中极具前景的优秀骨替代物。
