Department of Chemistry and the Centre for Advanced Nanomaterials , The University of Adelaide , Adelaide , South Australia 5005 , Australia.
Department of Chemistry, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials , Fudan University , Shanghai 200433 , P.R. China.
J Am Chem Soc. 2019 Feb 13;141(6):2348-2355. doi: 10.1021/jacs.8b10302. Epub 2019 Jan 30.
Encapsulation of biomacromolecules in metal-organic frameworks (MOFs) can preserve biological functionality in harsh environments. Despite the success of this approach, termed biomimietic mineralization, limited consideration has been given to the chemistry of the MOF coating. Here, we show that enzymes encapsulated within hydrophilic MAF-7 or ZIF-90 retain enzymatic activity upon encapsulation and when exposed to high temperatures, denaturing or proteolytic agents, and organic solvents, whereas hydrophobic ZIF-8 affords inactive catalase and negligible protection to urease.
将生物大分子封装在金属有机骨架（MOFs）中可以在恶劣环境中保持生物功能。尽管这种方法被称为仿生矿化取得了成功，但对 MOF 涂层的化学性质考虑有限。在这里，我们表明，亲水 MAF-7 或 ZIF-90 内包封的酶在包封后以及暴露于高温、变性或蛋白水解剂和有机溶剂时保留酶活性，而疏水性 ZIF-8 则提供无活性的过氧化氢酶和对脲酶的保护作用微不足道。
