Lee Hwa-Yong, Raveendar Sebastin, An Hyejin, Oh Youn-Lee, Jang Kab-Yeul, Kong Won-Sik, Ryu Hojin, So Yoon-Sup, Chung Jong-Wook
Department of Forest Science, Chungbuk National University, Cheongju, Republic of Korea.
National Agrobiodiversity Center, National Institute of Agricultural Science, RDA, Jeonju, Republic of Korea.
Mycobiology. 2018 Nov 28;46(4):421-428. doi: 10.1080/12298093.2018.1538072. eCollection 2018.
The white button mushroom () is one of the most widely cultivated species of edible mushroom. Despite its economic importance, relatively little is known about the genetic diversity of this species. Illumina paired-end sequencing produced 43,871,558 clean reads and 69,174 contigs were generated from five offspring. These contigs were subsequently assembled into 57,594 unigenes. The unigenes were annotated with reference genome in which 6,559 unigenes were associated with clusters, indicating orthologous genes. Gene ontology classification assigned many unigenes. Based on genome data of the five offspring, 44 polymorphic simple sequence repeat (SSR) markers were developed. The major allele frequency ranged from 0.42 to 0.92. The number of genotypes and the number of alleles ranged from 1 to 4, and from 2 to 4, respectively. The observed heterozygosity and the expected heterozygosity ranged from 0.00 to 1.00, and from 0.15 to 0.64, respectively. The polymorphic information content value ranged from 0.14 to 0.57. The genetic distances and UPGMA clustering discriminated offspring strains. The SSR markers developed in this study can be applied in polymorphism analyses of button mushroom and for cultivar discrimination.
白色双孢蘑菇（）是栽培最为广泛的食用蘑菇品种之一。尽管其具有重要的经济价值，但人们对该品种的遗传多样性了解相对较少。Illumina双末端测序产生了43,871,558条纯净 reads，从五个后代中生成了69,174个重叠群。这些重叠群随后被组装成57,594个单基因。单基因通过参考基因组进行注释，其中6,559个单基因与聚类相关，表明是直系同源基因。基因本体分类确定了许多单基因。基于五个后代的基因组数据，开发了44个多态性简单序列重复（SSR）标记。主要等位基因频率范围为0.42至0.92。基因型数量和等位基因数量分别为1至4个和2至4个。观察到的杂合度和预期杂合度分别为0.00至1.00和0.15至0.64。多态信息含量值范围为0.14至0.57。遗传距离和UPGMA聚类区分了后代菌株。本研究开发的SSR标记可应用于双孢蘑菇的多态性分析和品种鉴别。
