An Hyejin, Jo Ick-Hyun, Oh Youn-Lee, Jang Kab-Yeul, Kong Won-Sik, Sung Jwa-Kyung, So Yoon-Sup, Chung Jong-Wook
Department of Industrial Plant Science and Technology, Chungbuk National University, Cheongju, Republic of Korea.
Department of Herbal Crop Research, Rural Development Administration, National Institute of Horticultural and Herbal Science, Eumseong, Republic of Korea.
Mycobiology. 2019 Sep 27;47(4):527-532. doi: 10.1080/12298093.2019.1667131. eCollection 2019.
We designed 170 new simple sequence repeat (SSR) markers based on the whole-genome sequence data of button mushroom (), and selected 121 polymorphic markers. A total of 121 polymorphic markers, the average major allele frequency (M) and the average number of alleles (N) were 0.50 and 5.47, respectively. The average number of genotypes (N), observed heterozygosity (H), expected heterozygosity (H), and polymorphic information content (PIC) were 6.177, 0.227, 0.619, and 0.569, respectively. Pearson's correlation coefficient showed that M was negatively correlated with N (-0.683), N (-0.600), H (-0.584), and PIC (-0.941). N, N, H and PIC were positively correlated with other polymorphic parameters except for M. UPGMA clustering showed that 26 accessions were classified into 3 groups, and each accession was differentiated. The 121 SSR markers should facilitate the use of molecular markers in button mushroom breeding and genetic studies.
我们基于双孢蘑菇的全基因组序列数据设计了170个新的简单序列重复（SSR）标记，并筛选出121个多态性标记。121个多态性标记的平均主等位基因频率（M）和平均等位基因数（N）分别为0.50和5.47。平均基因型数（N）、观察杂合度（H）、期望杂合度（He）和多态信息含量（PIC）分别为6.177、0.227、0.619和0.569。Pearson相关系数表明，M与N（-0.683）、He（-0.600）、H（-0.584）和PIC（-0.941）呈负相关。N、He、H和PIC与除M之外的其他多态性参数呈正相关。UPGMA聚类分析表明，26个菌株被分为3组，且每个菌株都有区别。这121个SSR标记将有助于双孢蘑菇育种和遗传研究中分子标记的应用。
