Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, 61357-831351, Iran.
Young Researchers and Elites Club, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Probiotics Antimicrob Proteins. 2020 Mar;12(1):224-235. doi: 10.1007/s12602-018-9510-z.
For investigating the possible effect of Lactobacillus casei on the immune status of Shabot, Tor grypus, 480 fish weighing 40 ± 10 g were randomly divided into four groups in triplicate. The first group (control) was fed on a commercial diet without probiotic supplementation. Other groups (A, B, and C) were received diet supplemented by different levels of L. casei, i.e., 5 × 10, 5 × 10, and 5 × 10 CFU g, respectively. The feeding trial was performed for 60 continuous days followed by 15 days as withdraw period, i.e., day 75. Serum immune responses were determined on days 0, 30, 60, and 75 of feeding trial. Gene expression in the kidneys was analyzed on days 0, 60, and 75 as well as following bacterial challenge. The results showed that dietary L. casei supplementation significantly (P < 0.05) increased serum lysozyme, serum bactericidal, complement, and respiratory burst activities as compared with control group, while it was observed that treated fish did not show any significant differences in immune responses on day 75 (P < 0.05). Results of gene expression (TNF-α, IL-1β, and IL-8) showed significant increases among different experimental times (P < 0.05). According to obtained results, it might be concluded that the feeding by L. casei could likely enhance the immune responses and gene expression in T. grypus. Treatments A and C were better than B, but the lower concentration is better as an economic viewpoints and biosecurity. Therefore, concentration A is suggested.
为了研究干酪乳杆菌对沙波氏兔鱼免疫状态的可能影响，将 480 条重 40±10g 的鱼随机分为 4 组，每组 3 个重复。第一组（对照组）饲喂不含益生菌的商业饲料。其他组（A、B 和 C）分别饲喂添加不同水平干酪乳杆菌的饲料，即 5×10、5×10 和 5×10 CFU/g。饲喂试验持续 60 天，随后进行 15 天的停药期，即第 75 天。在饲喂试验的第 0、30、60 和 75 天测定血清免疫反应。在第 0、60 和 75 天以及细菌攻毒后分析肾脏中的基因表达。结果表明，与对照组相比，日粮中添加干酪乳杆菌显著（P<0.05）提高了血清溶菌酶、血清杀菌、补体和呼吸爆发活性，而在第 75 天观察到处理鱼的免疫反应没有任何显著差异（P<0.05）。基因表达（TNF-α、IL-1β 和 IL-8）的结果表明，不同实验时间之间存在显著差异（P<0.05）。根据获得的结果，可以得出结论，干酪乳杆菌的饲喂可能增强沙波氏兔鱼的免疫反应和基因表达。处理 A 和 C 优于 B，但从经济观点和生物安全考虑，较低的浓度更好。因此，建议使用浓度 A。
