Institute of Aquaculture, University of Stirling, Stirling, UK.
J Appl Microbiol. 2011 Aug;111(2):266-77. doi: 10.1111/j.1365-2672.2011.05044.x. Epub 2011 Jun 2.
To study the antagonic affect of probiotic Pseudomonas M174 on the fish pathogen Flavobacterium psychrophilum.
The ability of Pseudomonas M174 to inhibit the growth of Fl. psychrophilum was examined in iron-sufficient and -deficient media. Possible siderophore production was also investigated. Antagonistic activity was confirmed in disease challenge experiments using a rainbow trout (Oncorhynchus mykiss) model. Adhesion of Pseudomonas M174 to fish surfaces and its ability to stimulate innate immunity was also investigated in vivo. Pseudomonas M174 antagonized Fl. psychrophilum and produced siderophores in vitro. In challenge experiments with Fl. psychrophilum, fish fed with Pseudomonas M174 had lower levels of mortalities than the controls. It was possible to find Pseudomonas M174 in the intestinal content of these fish after feeding and bathing with the probiotic, but probiotic was obtained from the gills only after feeding. Respiratory burst activity was also found to be enhanced in the M174 fed fish.
These results suggest that M174 is a potential probiotic against Fl. psychrophilum and has several modes of action.
Probiotics are a promising alternative to the use of antibiotics in aquaculture and could be a more sustainable disease control method.
研究益生菌假单胞菌 M174 对鱼类病原体嗜冷杆菌的拮抗作用。
在铁充足和缺乏的培养基中检查了假单胞菌 M174 抑制嗜冷杆菌生长的能力。还研究了可能的铁载体产生情况。使用虹鳟（Oncorhynchus mykiss）模型进行疾病挑战实验证实了拮抗活性。还在体内研究了假单胞菌 M174 对鱼类表面的粘附能力及其刺激先天免疫的能力。假单胞菌 M174 拮抗嗜冷杆菌并在体外产生铁载体。在嗜冷杆菌的挑战实验中，与对照组相比，用假单胞菌 M174 喂养的鱼死亡率较低。在用益生菌喂养和沐浴后，可以在这些鱼的肠道内容物中找到假单胞菌 M174，但仅在喂食后才能从鱼鳃中获得益生菌。还发现 M174 喂养的鱼的呼吸爆发活性增强。
这些结果表明，M174 是一种针对嗜冷杆菌的潜在益生菌，具有多种作用方式。
益生菌是水产养殖中抗生素替代物的有前途的选择，可能是一种更可持续的疾病控制方法。
