Bianchi Matheus Viezzer, Silveira Simone, Mósena Ana Cristina Sbaraini, de Souza Suyene Oltramari, Konradt Guilherme, Canal Cláudio Wageck, Driemeier David, Pavarini Saulo Petinatti
Faculdade de Veterinária, Setor de Patologia Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9090, Porto Alegre, Rio Grande do Sul, 91540-000, Brazil.
Faculdade de Veterinária, Laboratório de Virologia, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9090, Porto Alegre, Rio Grande do Sul, 91540-000, Brazil.
Braz J Microbiol. 2019 Jan;50(1):271-277. doi: 10.1007/s42770-018-0019-0. Epub 2018 Nov 30.
Dermatitis might occur in mucosal disease (MD) caused by bovine viral diarrhea virus (BVDV). This study describes the pathological and virological features of skin lesions associated with BVDV infection in four persistently infected (PI) cattle. Skin samples were reprocessed for histopathology and IHC. BVDV isolates were obtained and were genetically characterized. In addition to upper alimentary system ulcerative lesions, all cattle (one outbreak and three individual cases) presented focal crusty and ulcerative lesions affecting the mucocutaneous and skin-horn junctions, interdigital clefts, pastern, and areas surrounding the dewclaws and diffuse thickened skin within 7-20 days of infection. Microscopic analysis revealed parakeratotic hyperkeratosis and single-cell keratinocyte death, accompanied by ballooning degeneration and spongiosis in the epidermis, as well as intraepithelial and subcorneal pustules. IHC showed BVDV antigen in the cytoplasm of keratinocytes undergoing individual cell death. Phylogenetic analysis revealed that the isolates from cattle #1, #2, and #4 belonged to BVDV-1a, whereas that from cattle #3 belonged to BVDV-1d. Cytopathic BVDV was isolated from cattle #2 and #3 (MD), and non-cytopathic BVDV was isolated from cattle #1 and #4. Thus, BVDV infection might cause acute disease, characterized by skin and upper alimentary system ulcerative lesions, in both MD and PI cattle.
牛病毒性腹泻病毒（BVDV）引起的黏膜病（MD）可能会出现皮炎。本研究描述了4头持续感染（PI）牛中与BVDV感染相关的皮肤病变的病理和病毒学特征。对皮肤样本进行重新处理以进行组织病理学和免疫组织化学（IHC）分析。获取BVDV分离株并进行基因特征分析。除了上消化道系统溃疡性病变外，所有牛（1头发病牛和3头个体病例）在感染后7 - 20天内均出现了影响黏膜皮肤和皮肤-角结合处、趾间裂、跗关节、悬蹄周围区域的局灶性结痂和溃疡性病变，以及弥漫性皮肤增厚。显微镜分析显示角化不全性角化过度和单细胞角质形成细胞死亡，伴有表皮气球样变性和海绵形成，以及上皮内和角质层下脓疱。免疫组织化学显示在发生单细胞死亡的角质形成细胞的细胞质中有BVDV抗原。系统发育分析表明，来自牛1、牛2和牛4的分离株属于BVDV - 1a，而来自牛3的分离株属于BVDV - 1d。从牛2和牛3（MD）中分离出细胞病变性BVDV，从牛1和牛4中分离出非细胞病变性BVDV。因此，BVDV感染可能会在MD牛和PI牛中引起以皮肤和上消化道系统溃疡性病变为特征的急性疾病。
