Department of Pharmacy, University of Split School of Medicine, Šoltanska 2, 21000, Split, Croatia.
Split-Dalmatia County Pharmacy, Kneza Ljudevita Posavskog 12 b, 21000, Split, Croatia.
Br J Dermatol. 2019 Jul;181(1):175-185. doi: 10.1111/bjd.17633. Epub 2019 Apr 11.
Sodium lauryl sulfate (SLS)-induced contact dermatitis is a commonly used model for testing the effects of different topical formulations. According to the guidelines, the volar forearms are the preferred testing site; however, other anatomical locations have been used in previous research, particularly the upper back as the clinically used site for testing different antigens.
To investigate the existence of anatomical variations of the skin response to irritation and its effects on the response to treatment.
Irritation was induced with SLS on symmetrical sites on both forearms and sides of the upper back with additional sites exposed to water as controls. Half of the sites were treated with emollient cream while the other half were left untreated. Irritation was assessed using bioengineering methods and clinical scoring. The trial was registered at ClinicalTrials.gov as NCT03231813.
Upper back skin showed higher reactivity to irritants with stronger barrier disruption (measured by Tewameter , 80·2 ± 18·3 vs. 48·0 ± 24·2 g m h ), more pronounced erythema (measured by Mexameter , 186·5 ± 88·4 vs. 92·1 ± 58·2 arbitrary units) and dryness (measured by Corneometer , -28·6 ± 14·5 vs. 2·7 ± 16·9 arbitrary units). Skin recovery rates were also influenced by anatomical location with the upper back showing faster recovery (316·7 ± 223·1 vs. 156·2 ± 198·5). Treatment did not lead to improvement in measured parameters, regardless of anatomical location.
The skin's reaction to irritant and recovery was dependent on anatomical location. The location where testing was conducted should always be reported, as treatments tested across different locations could not be directly compared with each other.
十二烷基硫酸钠（SLS）诱导的接触性皮炎是一种常用于测试不同局部制剂效果的模型。根据指南，首选测试部位是掌侧前臂；然而，以前的研究中也使用了其他解剖部位，特别是上背部，作为测试不同抗原的临床使用部位。
研究皮肤对刺激的反应在解剖部位上的差异及其对治疗反应的影响。
在双侧前臂和上背部两侧对称部位用 SLS 诱导刺激，并用对照水暴露其他部位。一半的部位用保湿霜治疗，另一半不治疗。使用生物工程方法和临床评分评估刺激。该试验在 ClinicalTrials.gov 上注册为 NCT03231813。
上背部皮肤对刺激物的反应性更高，屏障破坏更严重（通过 Tewameter 测量，80.2±18.3 vs. 48.0±24.2 g·m-2·h-1），红斑更明显（通过 Mexameter 测量，186.5±88.4 vs. 92.1±58.2 单位），干燥度更高（通过 Corneometer 测量，-28.6±14.5 vs. 2.7±16.9 单位）。皮肤恢复率也受到解剖部位的影响，上背部恢复更快（316.7±223.1 vs. 156.2±198.5）。无论解剖部位如何，治疗都不会导致测量参数的改善。
皮肤对刺激物的反应和恢复取决于解剖部位。应始终报告测试的位置，因为在不同位置进行的测试不能直接相互比较。
