Galderma R&D, Les Templiers, Sophia-Antipolis, France.
Paris, France.
Exp Dermatol. 2019 Mar;28(3):317-321. doi: 10.1111/exd.13880. Epub 2019 Feb 11.
Stratum corneum collected by tape stripping from 10 and 24 subjects with cutaneous T-cell lymphomas (CTCL) or psoriasis, respectively, were compared using quantitative label-free mass spectrometry analysis. A non-supervised statistical analysis (Posneg NMF) based on 352 differentially expressed proteins in both CTCL and psoriasis samples was able to separate the two disease groups and finally able to identify a set of 112 proteins that contributed most and significantly to the separation when compared to non-lesional samples. In addition, Luminex assay revealed that the increase in the amount of chemokines related to the inflammatory response, and immune cell infiltration and recruitment in lesional stratum corneum in CTCL, including CXCL8, CXCL9, CXCL10, CCL27, TNF and sICAM-1 was in agreement with published data on entire skin biopsies. Proteome analysis using quantitative methods including mass spectrometry and Luminex technology offered the possibility to investigate the relevant protein signature in CTCL and may be helpful to diagnose and investigate the efficacy of treatments in clinical investigations using non-invasive methods in future.
通过胶带剥离分别从患有皮肤 T 细胞淋巴瘤（CTCL）或银屑病的 10 名和 24 名受试者中收集的角质层，使用定量无标记质谱分析进行了比较。基于 CTCL 和银屑病样本中 352 个差异表达蛋白的无监督统计分析（Posneg NMF）能够分离出这两个疾病组，最终能够确定一组 112 个蛋白，与非病变样本相比，这些蛋白对分离的贡献最大且最显著。此外，Luminex 检测显示，与炎症反应、免疫细胞浸润和募集相关的趋化因子在 CTCL 病变角质层中的含量增加，包括 CXCL8、CXCL9、CXCL10、CCL27、TNF 和 sICAM-1，这与整个皮肤活检的已发表数据一致。使用包括质谱和 Luminex 技术在内的定量方法进行蛋白质组分析提供了研究 CTCL 中相关蛋白质特征的可能性，并可能有助于在未来使用非侵入性方法进行临床研究中诊断和研究治疗效果。
