Wouters Yannick, Keyaerts Els, Rector Annabel, Van Even Ellen, Vissers Steven, Koletzki Diana, Pattery Theresa, Rousseau Els, Van Ranst Marc, Laffut Wim
Department of Microbiology, Heilig Hart Hospital Lier, Mechelsestraat 24, 2500 Lier, Belgium.
Laboratory of Clinical and Epidemiological Virology, Rega Institute for Medical Research, KU Leuven, Herestraat 49, 3000 Leuven, Belgium.
Diagn Microbiol Infect Dis. 2019 May;94(1):33-37. doi: 10.1016/j.diagmicrobio.2018.11.022. Epub 2018 Dec 4.
The objective of this study was to compare the performance of the Idylla™ Respiratory (IFV-RSV) panel to the GeneXpert Xpert® Flu/RSV assay and establish the performance of a midturbinate swab compared to nasopharyngeal sampling. Considering GeneXpert® assay as imperfect reference standard, a positive percentage agreement between both assays of 98-100% for influenza A and 96-99% for influenza B could be calculated when 354 nasopharyngeal and 325 midturbinate swabs were retrospectively analyzed. Comparing midturbinate samples to nasopharyngeal specimens of 321 subjects, positive percentage agreement varied from 42% to 94% depending on both target virus and assay used. Negative percentage agreements ranged from 98% to 100% for both methods and sample type comparison. The Idylla™ assay showed excellent performance compared to the GeneXpert® assay for the detection of influenza virus. The study also showed a slightly better performance for nasopharyngeal sampling compared to the use of a midturbinate swab.
本研究的目的是比较Idylla™呼吸道（IFV-RSV）检测板与GeneXpert Xpert®流感/呼吸道合胞病毒检测的性能，并确定中鼻甲拭子与鼻咽采样相比的性能。将GeneXpert®检测视为不完善的参考标准，在对354份鼻咽拭子和325份中鼻甲拭子进行回顾性分析时，两种检测方法对甲型流感的阳性百分比一致性为98%-100%，对乙型流感为96%-99%。在比较321名受试者的中鼻甲样本与鼻咽标本时，根据目标病毒和所用检测方法的不同，阳性百分比一致性在42%至94%之间。两种方法和样本类型比较的阴性百分比一致性在98%至100%之间。与GeneXpert®检测相比，Idylla™检测在流感病毒检测方面表现出色。该研究还表明，与使用中鼻甲拭子相比，鼻咽采样的性能略好。
