比较六种样本到答案的流感 A/B 和呼吸道合胞病毒核酸扩增检测方法，使用儿童呼吸道标本。
Comparison of Six Sample-to-Answer Influenza A/B and Respiratory Syncytial Virus Nucleic Acid Amplification Assays Using Respiratory Specimens from Children.
机构信息
Department of Pathology and Laboratory Medicine, Children's Mercy Hospital, Kansas City, Missouri, USA.
Department of Pathology and Laboratory Medicine, Saint Luke's Hospital, Kansas City, Missouri, USA.
出版信息
J Clin Microbiol. 2018 Oct 25;56(11). doi: 10.1128/JCM.00930-18. Print 2018 Nov.
The rapid and accurate detection of influenza A virus (FluA), influenza B virus (FluB), and respiratory syncytial virus (RSV) improves patient care. Sample-to-answer (STA) platforms based on nucleic acid amplification and detection of these viruses are simple, automated, and accurate. We compared six such platforms for the detection of FluA, FluB, and RSV: Cepheid GeneXpert Xpress Flu/RSV (Xpert), Hologic Panther Fusion Flu A/B/RSV (Fusion), Cobas influenza A/B & RSV (Liat), Luminex Aries Flu A/B & RSV (Aries), BioFire FilmArray respiratory panel (RP), and Diasorin Simplexa Flu A/B & RSV (Simplexa). Nasopharyngeal (NP) swab specimens ( = 225) from children previously tested by RP were assessed on these platforms. The results were compared to those of the Centers for Disease Control and Prevention (CDC)-developed real-time reverse transcription-PCR (rRT-PCR) assay for influenza A/B viruses and RSV. Subtyping for FluA and FluB was performed for discrepant analysis where applicable. The percent sensitivities/specificities for FluA detection were 100/100 (Fusion), 98.6/99.3 (Xpert), 100/100 (Liat), 98.6/100 (Aries), 98.6/100 (Simplexa), and 100/100 (RP). The percent sensitivities/specificities for FluB detection were 100/100 (Fusion), 97.9/99.4 (Xpert), 97.9/98.3 (Liat), 93.7/99.4 (Aries), 85.4/99.4 (Simplexa), and 95.8/97.7 (RP); and those for RSV detection were 98.1/99.4 (Xpert), 98.1/99.4 (Liat), 96.3/100 (Fusion), 94.4/100 (Aries), 87/94.4 (Simplexa), and 94.4/100 (RP). The 75 strains confirmed to be FluA included 29 pH1N1, 39 H3N2, 4 sH1N1, and 3 untyped strains. The 48 strains confirmed to be FluB included 33 strains of the Yamagata lineage, 13 of the Victoria lineage, 1 of both the Yamagata and Victoria lineages, and 1 of an unknown lineage. All six STA platforms demonstrated >95% sensitivity for FluA detection, while three platforms (Fusion, Xpert, and Liat) demonstrated >95% sensitivity for FluB and RSV detection.
快速准确地检测甲型流感病毒 (FluA)、乙型流感病毒 (FluB) 和呼吸道合胞病毒 (RSV) 可改善患者的护理。基于核酸扩增和这些病毒检测的样本至结果 (STA) 平台简单、自动化且准确。我们比较了六种用于检测 FluA、FluB 和 RSV 的此类平台：Cepheid GeneXpert Xpress Flu/RSV (Xpert)、Hologic Panther Fusion Flu A/B/RSV (Fusion)、Cobas influenza A/B & RSV (Liat)、Luminex Aries Flu A/B & RSV (Aries)、BioFire FilmArray 呼吸道面板 (RP) 和 Diasorin Simplexa Flu A/B & RSV (Simplexa)。先前使用 RP 进行检测的儿童的鼻咽 (NP) 拭子标本 (=225) 在这些平台上进行了评估。结果与疾病控制与预防中心 (CDC) 开发的用于流感 A/B 病毒和 RSV 的实时逆转录聚合酶链反应 (rRT-PCR) 检测进行了比较。对适用于差异分析的 FluA 和 FluB 进行了亚分型。FluA 检测的灵敏度/特异性百分比为 100/100 (Fusion)、98.6/99.3 (Xpert)、100/100 (Liat)、98.6/100 (Aries)、98.6/100 (Simplexa) 和 100/100 (RP)。FluB 检测的灵敏度/特异性百分比为 100/100 (Fusion)、97.9/99.4 (Xpert)、97.9/98.3 (Liat)、93.7/99.4 (Aries)、85.4/99.4 (Simplexa) 和 95.8/97.7 (RP)；RSV 检测的灵敏度/特异性百分比为 98.1/99.4 (Xpert)、98.1/99.4 (Liat)、96.3/100 (Fusion)、94.4/100 (Aries)、87/94.4 (Simplexa) 和 94.4/100 (RP)。确认的 75 株 FluA 包括 29 株 pH1N1、39 株 H3N2、4 株 sH1N1 和 3 株未分型株。确认的 48 株 FluB 包括 33 株 Yamagata 谱系、13 株 Victoria 谱系、1 株 Yamagata 和 Victoria 谱系以及 1 株未知谱系。所有六个 STA 平台对 FluA 的检测均表现出 >95%的灵敏度，而三个平台 (Fusion、Xpert 和 Liat) 对 FluB 和 RSV 的检测均表现出 >95%的灵敏度。
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