Centre for Marine Bioproducts Development (CMBD), College of Medicine and Public Health, Flinders University, GPO Box 2100, Adelaide 5001, South Australia, Australia; Medical Biotechnology, College of Medicine and Public Health, Flinders University, GPO Box 2100, Adelaide 5001, South Australia, Australia; Ministry of Higher Education in Saudi Arabia, King Faisal Hospital Street, Riyadh 11153, Saudi Arabia.
Discipline of Pharmacology, School of Medicine, Faculty of Health Sciences, The University of Adelaide, Adelaide, South Australia, Australia.
Int J Biol Macromol. 2019 Feb 1;122:255-264. doi: 10.1016/j.ijbiomac.2018.10.168. Epub 2018 Oct 25.
This study investigated the neuroprotective activities of five different fucoidan samples with different chemical compositions prepared from Fucus vesiculosus (FE, FF, and S) and Undaria pinnatifida (UE and UF) to determine if they reduced aggregation or cytotoxicity of Aβ in neuronal PC-12 cells. Only fucoidans S, UE, and UF showed anti-aggregation effects against Aβ, as determined using Thioflavin T (ThT) fluorometric fibrillisation kinetics and transmission electron microscopy (TEM) of fibril morphology. However, all five fucoidan samples reduced the cytotoxicity of both Aβ and hydrogen peroxide in neuronal PC-12 cells and demonstrated inhibition of apoptosis induced by Aβ. Three fucoidan samples (FF, UE and UF) showed significant activity in enhancing neurite outgrowth. Fucoidan from different seaweed sources and with varying chemical compositions demonstrate a range of neuroprotective activities that may have potential to alter Aβ neurotoxicity in Alzheimer's disease.
本研究调查了五种不同化学组成的褐藻糖胶样品（来源于泡叶藻（FE、FF 和 S）和裙带菜（UE 和 UF））的神经保护活性，以确定它们是否能减少神经元 PC-12 细胞中 Aβ的聚集或细胞毒性。只有褐藻糖胶 S、UE 和 UF 显示出对 Aβ的抗聚集作用，这是通过使用 Thioflavin T（ThT）荧光纤维形成动力学和纤维形态的透射电子显微镜（TEM）来确定的。然而，所有五种褐藻糖胶样品都降低了神经元 PC-12 细胞中 Aβ和过氧化氢的细胞毒性，并显示出对 Aβ诱导的细胞凋亡的抑制作用。三种褐藻糖胶样品（FF、UE 和 UF）显示出对神经突生长的显著促进作用。来自不同海藻来源和具有不同化学成分的褐藻糖胶表现出一系列的神经保护活性，可能有潜力改变阿尔茨海默病中的 Aβ神经毒性。
