Department of Biotechnology, Engineering School of Lorena, University of São Paulo, Lorena, Brazil.
Department of Biotechnology, Engineering School of Lorena, University of São Paulo, Lorena, Brazil.
Int J Biol Macromol. 2019 Apr 15;127:169-177. doi: 10.1016/j.ijbiomac.2019.01.038. Epub 2019 Jan 10.
Pullulan is a biopolymer used in food industry produced by Aureobasidium pullulans from starch. In the present work, sugarcane bagasse (SCB) hydrolysate was evaluated as an alternative substrate in fermentation process assisted by blue LED lights. The best fermentation conditions in Erlenmeyer flasks were 25.3 °C, stirring speed of 232 rpm and yeast extract concentration of 1.88 g/L, yielding 25.19 g/L of pullulan, that corresponded to yield of 0.48 g/g and 0.28 g/(L·h) of volumetric productivity. By using a column bubble photobioreactor, similar yield values were obtained. Thermal properties of the produced pullulan as glass transition (Tg) and melting (Tm) temperatures were 38 °C and 160 °C, respectively, which were similar to the corresponding values of commercial food grade pullulan. Therefore, SCB hydrolysate is a promising substrate to produce good quality pullulan (86% of purity) with application in food industry, besides to represent a new alternative for biorefineries.
出芽短梗霉从淀粉中生产的生物聚合物普鲁兰多糖被广泛应用于食品工业。在本工作中，我们评估了甘蔗渣水解物作为蓝 LED 光辅助发酵过程中的替代底物。在摇瓶中最佳发酵条件为 25.3°C、搅拌速度 232rpm 和酵母提取物浓度 1.88g/L，可得到 25.19g/L 的普鲁兰多糖，相应的产率为 0.48g/g 和 0.28g/(L·h)的比体积生产率。使用柱状鼓泡光生物反应器也可获得类似的产率值。所生产的普鲁兰多糖的热性能，玻璃化转变温度（Tg）和熔融温度（Tm）分别为 38°C 和 160°C，与商业食品级普鲁兰多糖的相应值相似。因此，甘蔗渣水解物是一种有前途的生产高质量普鲁兰多糖（纯度 86%）的底物，可应用于食品工业，此外还为生物精炼厂提供了一种新的替代选择。
