Usmanova Aizhamal, Brazhnikova Yelena, Omirbekova Anel, Kistaubayeva Aida, Savitskaya Irina, Ignatova Lyudmila
Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty 050038, Kazakhstan.
Scientific Research Institute of Biology and Biotechnology Problems, Al-Farabi Kazakh National University, Almaty 050038, Kazakhstan.
Polymers (Basel). 2024 Jan 30;16(3):376. doi: 10.3390/polym16030376.
Infections of agricultural crops caused by pathogen ic fungi are among the most widespread and harmful, as they not only reduce the quantity of the harvest but also significantly deteriorate its quality. This study aims to develop unique seed-coating formulations incorporating biopolymers (polyhydroxyalkanoate and pullulan) and beneficial microorganisms for plant protection against phytopathogens. A microbial association of biocompatible endophytic bacteria has been created, including D5, A2, B5, and D7. These strains exhibited agronomically valuable properties: synthesis of the phytohormone IAA (from 45.2 to 69.2 µg mL), antagonistic activity against and (growth inhibition zones from 1.8 to 3.0 cm), halotolerance (5-15% NaCl), and PHA production (2.77-4.54 g L). A pullulan synthesized by C7 showed a low viscosity rate (from 395 Pa·s to 598 Pa·s) depending on the concentration of polysaccharide solutions. Therefore, at 8.0%, / concentration, viscosity virtually remained unchanged with increasing shear rate, indicating that it exhibits Newtonian flow behavior. The effectiveness of various antifungal seed coating formulations has been demonstrated to enhance the tolerance of barley plants to phytopathogens.
由病原真菌引起的农作物感染是最为普遍且有害的，因为它们不仅会减少收成数量，还会显著降低其质量。本研究旨在开发独特的种子包衣配方，其中包含生物聚合物（聚羟基脂肪酸酯和普鲁兰多糖）以及有益微生物，用于保护植物免受植物病原体侵害。已创建了一种生物相容性内生细菌的微生物组合，包括D5、A2、B5和D7。这些菌株表现出具有农业价值的特性：植物激素IAA的合成（45.2至69.2 µg mL）、对[此处原文缺失两种病原体名称]的拮抗活性（生长抑制区为1.8至3.0 cm）、耐盐性（5 - 15% NaCl）以及PHA的产生（2.77至4.54 g L）。由C7合成的普鲁兰多糖根据多糖溶液浓度显示出较低的粘度率（395 Pa·s至598 Pa·s）。因此，在8.0%的[此处原文缺失两种物质名称]浓度下，随着剪切速率增加，粘度实际上保持不变，表明它表现出牛顿流体行为。已证明各种抗真菌种子包衣配方可提高大麦植株对植物病原体的耐受性。
