Muso Cachumba J J, Terán Hilares R, Brumano L P, Marcelino P R F, Antunes F A F, Santos J C, da Silva S S
a Department of Biotechnology, Engineering School of Lorena , University of São Paulo , Lorena , São Paulo , Brazil.
Prep Biochem Biotechnol. 2019;49(4):328-333. doi: 10.1080/10826068.2019.1566152. Epub 2019 Feb 3.
L-asparaginase is an important enzyme used in the pharmaceutical and food industry, which can be produced by different microorganisms using low cost feedstocks. In this work, sugarcane bagasse (SCB) was used as support for enzyme production in solid-state fermentation (SSF) by A. terreus. Initially, the influence of the variables carbon and nitrogen sources on the enzyme production was studied following an experimental design carried out in Erlenmeyer flasks. Statistical analysis indicated the use of 0.54% of starch, 0% of maltose, 0.44% of asparagine, and 1.14% of glutamine in the medium, resulting in enzyme activity per volume of produced extract of 120.723 U/L. Then, these conditions were applied in a horizontal column reactor filled with SCB, producing 105.3 U/L of enzyme activity. Therefore, the potential of extracellular L-asparaginase enzyme production in the column reactor using sugarcane bagasse as support was demonstrated and it represents a system that can favor large scale production.
L-天冬酰胺酶是制药和食品工业中使用的一种重要酶，可由不同微生物利用低成本原料生产。在这项工作中，甘蔗渣被用作土曲霉固态发酵（SSF）产酶的载体。最初，按照在锥形瓶中进行的实验设计，研究了碳源和氮源变量对酶生产的影响。统计分析表明，培养基中使用0.54%的淀粉、0%的麦芽糖、0.44%的天冬酰胺和1.14%的谷氨酰胺，每升所产提取物的酶活性为120.723 U/L。然后，将这些条件应用于装有甘蔗渣的水平柱式反应器中，酶活性为105.3 U/L。因此，证明了以甘蔗渣为载体在柱式反应器中生产细胞外L-天冬酰胺酶的潜力，它代表了一个有利于大规模生产的系统。
