Parvez Rehnuma, Vijayakumar Santhiya, Vins Alwin, Ramaiah Sudha, Anbarasu Anand, Biswas Lipika, Beniwal Nisha, Kaur Harpreet, Muruganandam Nagarajan
Indian Council of Medical Research - Regional Medical Research Centre, Port Blair, Andaman and Nicobar Islands, India.
Department of Biosciences, School of Biosciences and Technology (SBST), Vellore Institute of Technology (VIT), Vellore, India.
Front Cell Infect Microbiol. 2025 Jan 15;14:1486166. doi: 10.3389/fcimb.2024.1486166. eCollection 2024.
Human papillomavirus (HPV) is a viral infection, and its acquisition and persistence are significantly influenced by the vaginal microbiota. Understanding and comparing the vaginal microbiome of HPV infected women in Andaman and Nicobar Islands is crucial.
The study involved collecting vaginal swabs and extracting DNA using the QIAamp DNA Minikit. The DNA was then subjected to PCR amplification to confirm HPV infection. illumina NovaSeq 6000 platform was utilized to perform sequencing utilizing 2 x 250 paired end chemistry. Taxonomic analysis was performed and Bacterial abundance plots were generated and samples were grouped based on demographic parameters, pap test diagnosis, and genotypes. To assess diversity, samples were rarefied to 49,000 sequence reads per sample, and alpha and beta diversity metrics were calculated.
The study analyzed the presence of 21 assigned phyla, with Firmicutes, Actinobacteria, Bacteriodetes, and Proteobacteria emerging as the predominant taxa. At the genus level, and dominated across all samples. was significantly more abundant in HPV-positive (22.40%) compared to HPV-negative samples (10.04%). Symptomatic group of HPV-positive samples had , and unclassified Coriobacteriaceae being dominant. In terms of bacterial diversity, the study found statistically significant association when comprising individuals aged 21 to 30 years to those aged 31 to 40 years.
Most research concluded that exposure to HPV can boost bacterial diversity in vagina compared to healthy women, increasing the risk of cervical cancer development. Current study highlights the importance of vaginal microbiome associated with high and low risk HPV, various age group as well as the symptomatic and asymptomatic cases of HPV infected women in South Andaman.
人乳头瘤病毒（HPV）是一种病毒感染，其感染和持续存在受阴道微生物群的显著影响。了解和比较安达曼和尼科巴群岛HPV感染女性的阴道微生物组至关重要。
该研究包括收集阴道拭子，并使用QIAamp DNA微量试剂盒提取DNA。然后对DNA进行PCR扩增以确认HPV感染。利用Illumina NovaSeq 6000平台，采用2×250双端化学法进行测序。进行分类分析，生成细菌丰度图，并根据人口统计学参数、巴氏试验诊断和基因型对样本进行分组。为了评估多样性，将样本稀疏到每个样本49000个序列读数，并计算α和β多样性指标。
该研究分析了21个指定门的存在情况，厚壁菌门、放线菌门、拟杆菌门和变形菌门是主要的分类群。在属水平上， 和 在所有样本中占主导地位。与HPV阴性样本（10.04%）相比，HPV阳性样本中 明显更为丰富（22.40%）。HPV阳性样本的症状组中， 和未分类的科里杆菌科占主导地位。在细菌多样性方面，该研究发现，年龄在21至30岁的个体与年龄在31至40岁的个体之间存在统计学上的显著关联。
大多数研究得出结论，与健康女性相比，接触HPV会增加阴道细菌多样性，增加患宫颈癌的风险。当前研究强调了安达曼南部与高危和低危HPV相关的阴道微生物组的重要性，以及HPV感染女性的不同年龄组以及有症状和无症状病例。
