School of Information and Communication Engineering, Hubei University of Economics, Wuhan 430205, China.
The Institute for Systems Biology, Jianghan University, Wuhan 430056, China.
Genomics. 2019 Dec;111(6):1966-1976. doi: 10.1016/j.ygeno.2019.01.008. Epub 2019 Jan 11.
Histone acetylation is an important post-translational modification in eukaryotes and is regulated by two antagonistic enzymes, namely histone acetyltransferase (HAT) and histone deacetylase (HDAC). However, little has been done on the HAT superfamily in Brachypodium distachyon (B. distachyon), a new model plant of Poaceae. In this study, eight HATs were identified from B. distachyon and classified into four major families. Subcellular localization analysis showed that a majority of BdHATs were predominantly localized in the nucleus. Syntenic and phylogenetic analysis indicated there may be two common ancestral CREB-binding protein (p300/CBP, HAC) genes prior to the separation of monocots and dicots. Expression analysis revealed that the potential roles of BdHATs in B. distachyon development and responses to four abiotic stresses. Protein-protein network analysis identified some potential interactive genes with BdHATs. Thus, our results will provide solid basis for further study the function of HAT genes in B. distachyon and other monocot plants.
组蛋白乙酰化是真核生物中一种重要的翻译后修饰，受两种拮抗酶的调节，即组蛋白乙酰转移酶（HAT）和组蛋白去乙酰化酶（HDAC）。然而，在新型禾本科模式植物短柄草（Brachypodium distachyon，B. distachyon）中，HAT 超家族的研究还很少。本研究从 B. distachyon 中鉴定了 8 个 HAT，并将其分为四个主要家族。亚细胞定位分析表明，大多数 BdHAT 主要定位于细胞核。共线性和系统发育分析表明，在单子叶植物和双子叶植物分化之前，可能存在两个共同的 CREB 结合蛋白（p300/CBP，HAC）基因的祖先。表达分析显示，BdHATs 在 B. distachyon 发育和对四种非生物胁迫响应中的潜在作用。蛋白质-蛋白质网络分析鉴定了一些与 BdHATs 具有潜在相互作用的基因。因此，我们的研究结果将为进一步研究 HAT 基因在 B. distachyon 和其他单子叶植物中的功能提供坚实的基础。
