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生物柴油粗甘油预处理毛竹提高生物乙醇产量:基质消化率、纤维素酶吸附和发酵性能。

Enhancement of bioethanol production from Moso bamboo pretreated with biodiesel crude glycerol: Substrate digestibility, cellulase absorption and fermentability.

机构信息

Department of Chemistry and Chemical Engineering, MOE Engineering Research Center of Forestry Biomass Materials and Bioenergy, Beijing Forestry University, Beijing 100083, China.

GuangXi Key Laboratory of Chemistry and Engineering of Forest Products, College of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, China.

出版信息

Bioresour Technol. 2019 Mar;276:300-309. doi: 10.1016/j.biortech.2019.01.017. Epub 2019 Jan 7.

Abstract

Utilization of sustainable energy is limited by energy requirement for the manufacturing of renewable fuels. Moso bamboo was pretreated with industrially derived crude glycerol obtained from different sources at 150/160 °C for 3 h. This bamboo, pretreated with base biodiesel glycerol with pressure filtration removal method, showed a high glucose yield of 94.95% and an ethanol yield of 73.10% of the theoretical. Major glycerol content was removed by pressure filtration, leaving a small amount of fatty acid soap in the pretreated sample, which formed an emulsion that reduced lignin redisposition onto the biomass surface and effectively blocked lignin absorption of cellulase, allowing greater enzymatic hydrolysis and fermentation system function. The surface was more hydrophilic and a higher lignin removal was achieved: 39.24% with base biodiesel glycerol pretreatment compared to 26.08% with sodium hydroxide glycerol pretreatment. This study provides a useful and cost-effective process, BBGP, for high-yield ethanol production.

摘要

利用可持续能源受到制造可再生燃料所需能源的限制。毛竹在 150/160°C 下用工业衍生的粗甘油处理 3 小时,粗甘油来自不同的来源。用碱性生物柴油甘油预处理并采用加压过滤去除法,得到的葡萄糖产率为 94.95%,理论乙醇产率为 73.10%。加压过滤去除了大部分甘油,使预处理样品中仅残留少量脂肪酸皂,形成乳液,减少了木质素重新分布到生物质表面的情况,有效地阻止了纤维素酶对木质素的吸收,使更多的酶解和发酵系统功能得以发挥。表面更亲水,木质素去除率更高:用碱性生物柴油甘油预处理可达到 39.24%,而用氢氧化钠甘油预处理则为 26.08%。本研究提供了一种有用且具有成本效益的工艺,即 BBGP,可用于高产乙醇的生产。

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